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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

CN037_MOUSE

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CN037_MOUSE N/A
Sequence Length (AA) Molecular Weight (Da)
775 83774
Protein Name
Uncharacterized protein C14orf37 homolog
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLQDSITGIV NSFNLFFPST MSRPTLMPTC VAFCSILFLT LATGCQAFPK
60 70 80 90 100
VERRETAQEY AEKEQSQKMN TDDQENISFA PKYMLQQMSS EAPMVLSEGP
110 120 130 140 150
SEIPLIKVFS VNKESHLPGA GLLHPTSPGV YSSSEPVVSA SEQEPGPSLL
160 170 180 190 200
ERMSSEHSLS KVMLTVAVSS PASLNPDQEG PYNSLSTQPI VAAVTDVTHG
210 220 230 240 250
SLDYLDNQLF AAKSQEAVSL GNSPSSSINT KEPEIIKADA AMGTTVVPGV
260 270 280 290 300
DSTGDMEPDR ERPSEMAADD GQSTTTKYLV TIPNNFLTTE PTAGSILGDA
310 320 330 340 350
KVTVSVSTAG PVSSIFNEEW DDTKFESISR GRPPEPGDNA ETQMRTKPPH
360 370 380 390 400
GTYESFEGTE ESPSSTAVLK VAPGHLGGEP ALGTALVTAL GDERSPVLTH
410 420 430 440 450
QISFTPMSLA EDPEVSTMKL FPSAGGFRAS TQGDRTQLSS ETAFSTSQYE
460 470 480 490 500
SVPQQEAGNV LKDITQERKM ATQAMNTTSP VVTQEHMATI EVPRGSGEPE
510 520 530 540 550
EGMPSLSPVP AEVADAELSR RGESLATPAS TTVVPLSLKL TSSMEDLMDT
560 570 580 590 600
ITGPSEEFIP VLGSPMAPPA MTVEAPTISS ALPSEGRTSP SISRPNTAAS
610 620 630 640 650
YGLEQLESEE VEDDEDEEDE EDEEEEEEDE EDEEDEEDKE TDSLYKDFDG
660 670 680 690 700
DTEPPGFTLP GITSQEPDIR SGSMDLLEVA TYQVPETIEW EQQNQGLVRS
710 720 730 740 750
WMEKLKDKAG YMSGMLVPVG VGIAGALFIL GALYSIKVMN RRRRNGFKRH
760 770 775
KRKQREFNSM QDRVMLLADS SEDEF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3453 Collapse assay details

Data source: Panorama

Official Gene Symbol
CN037_MOUSE
Peptide Sequence
LFPSAGGFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
420
Peptide End
428
CPTAC ID
non-CPTAC-3453
Peptide Molecular Mass
950.4974
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.6 3.5 2.9 12.2 4.9 5.5 13.9 6 6.2 15 15 15
y6 (1+) 10.2 4.2 3.1 10.6 5.6 4.2 14.7 7 5.2 15 15 15
y8 (2+) 29.3 11.5 6.5 26.9 11.5 6.4 39.8 16.3 9.1 15 15 15
y7 (2+) 6.3 2.1 2.3 10.7 5.5 4.8 12.4 5.9 5.3 15 15 15
b2 (1+) 8.6 4.6 3 7.8 5.3 2.8 11.6 7 4.1 15 15 15
sum 4.1 1.6 1.7 8.7 4.7 4.3 9.6 5 4.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments