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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Myrf Gm1804, Gm98, Mrf
Sequence Length (AA) Molecular Weight (Da)
1138 123288
Protein Name
Myelin regulatory factor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEVVDETEAL QRFFEGHDIS GALEPSNIDT SILEEYIGKE DASDLCFPEI
60 70 80 90 100
SAPASTASFP HGPPAIPGSS GLHHLSPPGS GPSPGRHGPL PPPTYGTPLN
110 120 130 140 150
CNNNNGMGTA PKPFLGGSGP PIKAEPKAPY APGTLPDSPP DSGSEAYSPQ
160 170 180 190 200
QVNDPHLLRT ITPETLCHVG VSSRLEHPPP PPAHLPGPPP PPPPPPHYPV
210 220 230 240 250
LQRDLYMKAE PPVPPYAAMG PGLVPPELHH TQQTQVLHQL LQQHGAELPP
260 270 280 290 300
HPSKKRKHSE SPPNTLNAQM LNGMIKQEPG TVTALPPHPA RAPSPPWPPQ
310 320 330 340 350
GPLSPGTGSL PLSIARAQTP PWHPPGAPSP GLLQDSDSLS GSYLDPNYQS
360 370 380 390 400
IKWQPHQQNK WATLYDANYK ELPMLTYRVD ADKGFNFSVG DDAFVCQKKN
410 420 430 440 450
HFQVTVYIGM LGEPKYVKTP EGLKPLDCFY LKLHGVKLEA LNQSINIEQS
460 470 480 490 500
QSDRSKRPFN PVTVNLPPEQ VTKVTVGRLH FSETTANNMR KKGKPNPDQR
510 520 530 540 550
YFMLVVALQA HAQNQNYTLA AQISERIIVR ASNPGQFESD SDVLWQRAQL
560 570 580 590 600
PDTVFHHGRV GINTDRPDEA LVVHGNVKVM GSLMHPSDLR AKEHVQEVDT
610 620 630 640 650
TEQLKRISRM RLVHYRYKPE FAASAGIEAT APETGVIAQE VKEILPEAVK
660 670 680 690 700
DTGDVVFANG KTIENFLVVN KERIFMENVG AVKELCKLTD NLETRIDELE
710 720 730 740 750
RWSHKLAKLR RLDSLKSTGS SGAFSHAGSQ FSRAGSVPHK KRPPKLANKS
760 770 780 790 800
SPAVPDQACI SQRFLQGTII ALVVVMAFSV VSMSTLYVLS LRSEEDLVDA
810 820 830 840 850
DGSLAVSTSC LLALLRPQDP GGSEAMCPWS SQSFGTTQLR QSSMTTGLPG
860 870 880 890 900
TQPSLLLVTK SASGPALRAL DLCSSQPCPI VCCSPPVSSP ATDPALGPTL
910 920 930 940 950
TPTPSPSSNP KHSGPGQMAP LPVTNIRAKS WGISANGISY SKHSKSLEPL
960 970 980 990 1000
ASPVVPFPGG QSKTKNSPSF NLQSRARRGA PQPSPSPAQF TQTQGQLDPA
1010 1020 1030 1040 1050
PSLTSIQLLE NSMPITSQYC VPEGACRLGN FTYHIPVSSS TPLHLSLTLQ
1060 1070 1080 1090 1100
MNSSTPVSVV LCSLTSEEEP CEEGGFLQRF HPHQDTQGTS HQWPVTILSF
1110 1120 1130 1138
REFTYHFRVT LLGQANCSSE AIVQPATDYY FHFYRLCD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3480 Collapse assay details

Data source: Panorama

Official Gene Symbol
Myrf
Peptide Sequence
AQLPDTVFHHGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
548
Peptide End
559
CPTAC ID
non-CPTAC-3480
Peptide Molecular Mass
1,376.6949
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 3.5 3.8 5.1 7 6.5 6.6 7.8 7.5 8.3 15 15 15
y9 (2+) 4.8 4.9 4.4 5.9 5.2 4 7.6 7.1 5.9 15 15 15
y10 (2+) 6.7 3.5 3.5 10.4 4.6 5.7 12.4 5.8 6.7 15 15 15
y3 (1+) 10.8 6.3 3.5 11.1 7.1 5.8 15.5 9.5 6.8 15 15 15
y5 (1+) 6.5 5.8 3.9 8.6 6.7 4.9 10.8 8.9 6.3 15 15 15
sum 4 2.7 1.5 7 4.3 3.7 8.1 5.1 4 15 15 15


Additional Resources and Comments