Mmp12, CPTAC-3481 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Mmp12	Mme, Mmel

Sequence Length (AA)	Molecular Weight (Da)
473	54971

Protein Name
Macrophage metalloelastase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSCTLLKGVC	TMKFLMMIVF	LQVSACGAAP	MNDSEFAEWY	LSRFYDYGKD
60	70	80	90	100
RIPMTKTKTN	RNFLKEKLQE	MQQFFGLEAT	GQLDNSTLAI	MHIPRCGVPD
110	120	130	140	150
VQHLRAVPQR	SRWMKRYLTY	RIYNYTPDMK	REDVDYIFQK	AFQVWSDVTP
160	170	180	190	200
LRFRKLHKDE	ADIMILFAFG	AHGDFNYFDG	KGGTLAHAFY	PGPGIQGDAH
210	220	230	240	250
FDEAETWTKS	FQGTNLFLVA	VHELGHSLGL	QHSNNPKSIM	YPTYRYLNPS
260	270	280	290	300
TFRLSADDIR	NIQSLYGAPV	KPPSLTKPSS	PPSTFCHQSL	SFDAVTTVGE
310	320	330	340	350
KIFFFKDWFF	WWKLPGSPAT	NITSISSIWP	SIPSGIQAAY	EIESRNQLFL
360	370	380	390	400
FKDEKYWLIN	NLVPEPHYPR	SIYSLGFSAS	VKKVDAAVFD	PLRQKVYFFV
410	420	430	440	450
DKHYWRYDVR	QELMDPAYPK	LISTHFPGIK	PKIDAVLYFK	RHYYIFQGAY
460	470	473
QLEYDPLFRR	VTKTLKSTSW	FGC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3481 Collapse assay details

Data source: Panorama

Official Gene Symbol: Mmp12
Peptide Sequence: LSADDIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 254
Peptide End: 260

CPTAC ID: non-CPTAC-3481
Peptide Molecular Mass: 788.4028
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	5.5	3.9	4.9	23.7	12.9	9.6	24.3	13.5	10.8	15	15	15
y2 (1+)	7.1	5	6.7	19.1	14.7	9.6	20.4	15.5	11.7	15	15	15
y4 (1+)	21.1	10	7.5	23.8	13.2	8	31.8	16.6	11	15	15	15
y5 (1+)	8.6	6	4.4	15.9	14.1	9	18.1	15.3	10	15	15	15
y6 (1+)	7.4	6	7.9	13.9	13.7	14.4	15.7	15	16.4	15	15	15
sum	3.6	2.8	2.2	15.8	13	8.9	16.2	13.3	9.2	15	15	15

Mmp12

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3481 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Mmp12

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3481 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments