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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Mmp12 Mme, Mmel
Sequence Length (AA) Molecular Weight (Da)
473 54971
Protein Name
Macrophage metalloelastase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSCTLLKGVC TMKFLMMIVF LQVSACGAAP MNDSEFAEWY LSRFYDYGKD
60 70 80 90 100
RIPMTKTKTN RNFLKEKLQE MQQFFGLEAT GQLDNSTLAI MHIPRCGVPD
110 120 130 140 150
VQHLRAVPQR SRWMKRYLTY RIYNYTPDMK REDVDYIFQK AFQVWSDVTP
160 170 180 190 200
LRFRKLHKDE ADIMILFAFG AHGDFNYFDG KGGTLAHAFY PGPGIQGDAH
210 220 230 240 250
FDEAETWTKS FQGTNLFLVA VHELGHSLGL QHSNNPKSIM YPTYRYLNPS
260 270 280 290 300
TFRLSADDIR NIQSLYGAPV KPPSLTKPSS PPSTFCHQSL SFDAVTTVGE
310 320 330 340 350
KIFFFKDWFF WWKLPGSPAT NITSISSIWP SIPSGIQAAY EIESRNQLFL
360 370 380 390 400
FKDEKYWLIN NLVPEPHYPR SIYSLGFSAS VKKVDAAVFD PLRQKVYFFV
410 420 430 440 450
DKHYWRYDVR QELMDPAYPK LISTHFPGIK PKIDAVLYFK RHYYIFQGAY
460 470 473
QLEYDPLFRR VTKTLKSTSW FGC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3481 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mmp12
Peptide Sequence
LSADDIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
254
Peptide End
260
CPTAC ID
non-CPTAC-3481
Peptide Molecular Mass
788.4028
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.5 3.9 4.9 23.7 12.9 9.6 24.3 13.5 10.8 15 15 15
y2 (1+) 7.1 5 6.7 19.1 14.7 9.6 20.4 15.5 11.7 15 15 15
y4 (1+) 21.1 10 7.5 23.8 13.2 8 31.8 16.6 11 15 15 15
y5 (1+) 8.6 6 4.4 15.9 14.1 9 18.1 15.3 10 15 15 15
y6 (1+) 7.4 6 7.9 13.9 13.7 14.4 15.7 15 16.4 15 15 15
sum 3.6 2.8 2.2 15.8 13 8.9 16.2 13.3 9.2 15 15 15

Additional Resources and Comments