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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Pros1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Pros1 Pros
Sequence Length (AA) Molecular Weight (Da)
675 74934
Protein Name
Vitamin K-dependent protein S
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRVLSARFRV LLACLALVIP VSETNFLSKE RASQVLVRKR RANTLFEETM
60 70 80 90 100
KGNLERECIE ELCNKEEARE VFENNPETDY FYPKYLGCLG AFRVGSFHAA
110 120 130 140 150
RQSANAYPDL RSCVKAISDQ CDPIPCNEDG YLACQDGQAA FTCFCKPGWQ
160 170 180 190 200
GDRCQYDVNE CKDPSNVNGG CSQICDNTPG SYHCSCKRGF AMLPNKKDCK
210 220 230 240 250
DLDECALKPS VCGTAVCKNI PGDFECECPD GYRYDPSSKS CKDVDECSEN
260 270 280 290 300
MCAQLCVNFP GGYSCYCDGK KGFKLAQDQK SCEGIPVCLS LDLDKNYELL
310 320 330 340 350
YLAEQFAGVV LYLKFRLPDI TRFSAEFDFR TYDSEGIILY AESLDHSNWL
360 370 380 390 400
LIALRDGKIE VQFKNEFSTQ ITTGGNVINN GIWNMVSVEE LDDSVSIKIA
410 420 430 440 450
KEAVMNINKL GSLFKPTDGF LDTKIYFAGL PRKVESALIK PINPRLDGCI
460 470 480 490 500
RGWNLMKQGA LGAKEIIEGK QNKHCFLNVE KGSYYPGSGI AQFSIDYNNV
510 520 530 540 550
TNAEGWQMNV TLNIRPSTGT GVMLALVSGG TVPFALSLVD SRSGTSQDIV
560 570 580 590 600
VFVENSVVAR LEAVSLCSDQ QSQLKCNVNR NGLELWTPLR KDVIYSKDLQ
610 620 630 640 650
RQLAVLDKAM KRTVATYLGG IPDISFSATP VNAFYSGCME VNINGVQLDL
660 670 675
DEAISKHKDI RAHSCPSVRK IQKNF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3543 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pros1
Peptide Sequence
FSAEFDFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
323
Peptide End
330
CPTAC ID
non-CPTAC-3543
Peptide Molecular Mass
1,017.4556
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 36.2 9.5 13.3 36.8 20.5 16.3 51.6 22.6 21 15 15 15
b2 (1+) 8.1 6.8 4.4 16.6 11.2 6.2 18.5 13.1 7.6 15 15 15
y2 (1+) 6.4 5.7 6.8 13.1 5.6 9.9 14.6 8 12 15 15 15
y6 (1+) 4.1 4.5 7.6 12.7 6.2 10.6 13.3 7.7 13 15 15 15
y7 (1+) 12.8 7 7.5 14.1 7.8 11.5 19 10.5 13.7 15 15 15
sum 4.4 2.8 3.8 11.4 4 7.8 12.2 4.9 8.7 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for non-CPTAC-2705 Collapse assay details

Data source: Panorama

Official Gene Symbol
PROS1
Peptide Sequence
SFQTGLFTAAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
91
Peptide End
101
CPTAC ID
non-CPTAC-2705
Peptide Molecular Mass
1,197.6142
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.9 5.9 6.1 8.8 7.4 7.8 10.6 9.5 9.9 15 15 15
y4 (1+) 6.3 6.4 9.1 12.5 8.6 10.3 14 10.7 13.7 15 15 15
y9 (2+) 11.1 5.3 6.3 17.3 8.4 9 20.6 9.9 11 15 15 15
y8 (1+) 5 10.5 8.3 12 11.2 10.8 13 15.4 13.6 15 15 15
y7 (1+) 8.1 6.9 11.2 10.9 9.6 10.9 13.6 11.8 15.6 15 15 15
sum 3.3 4.1 4.4 9.1 6.5 7.3 9.7 7.7 8.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3934 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pros1
Peptide Sequence
VGSFHAAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
94
Peptide End
101
CPTAC ID
non-CPTAC-3934
Peptide Molecular Mass
843.4351
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 6.8 9.5 11.8 13.3 8.5 13.2 14.9 12.7 17.7 15 15 15
y4 (1+) 10 5 17.5 13.8 9.7 18.7 17 10.9 25.6 15 15 15
y7 (1+) 16.4 10.2 10.3 16.1 11 10.1 23 15 14.4 15 15 15
y4 (2+) 14.2 8.9 10 13.7 14.9 9.6 19.7 17.4 13.9 15 15 15
y7 (2+) 17.7 11.6 7.3 17.1 10.2 11.3 24.6 15.4 13.5 15 15 15
sum 6.9 4.6 2.6 6.5 5.2 5.1 9.5 6.9 5.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments