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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Csf1r Csfmr, Fms
Sequence Length (AA) Molecular Weight (Da)
977 109179
Protein Name
Macrophage colony-stimulating factor 1 receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MELGPPLVLL LATVWHGQGA PVIEPSGPEL VVEPGETVTL RCVSNGSVEW
60 70 80 90 100
DGPISPYWTL DPESPGSTLT TRNATFKNTG TYRCTELEDP MAGSTTIHLY
110 120 130 140 150
VKDPAHSWNL LAQEVTVVEG QEAVLPCLIT DPALKDSVSL MREGGRQVLR
160 170 180 190 200
KTVYFFSPWR GFIIRKAKVL DSNTYVCKTM VNGRESTSTG IWLKVNRVHP
210 220 230 240 250
EPPQIKLEPS KLVRIRGEAA QIVCSATNAE VGFNVILKRG DTKLEIPLNS
260 270 280 290 300
DFQDNYYKKV RALSLNAVDF QDAGIYSCVA SNDVGTRTAT MNFQVVESAY
310 320 330 340 350
LNLTSEQSLL QEVSVGDSLI LTVHADAYPS IQHYNWTYLG PFFEDQRKLE
360 370 380 390 400
FITQRAIYRY TFKLFLNRVK ASEAGQYFLM AQNKAGWNNL TFELTLRYPP
410 420 430 440 450
EVSVTWMPVN GSDVLFCDVS GYPQPSVTWM ECRGHTDRCD EAQALQVWND
460 470 480 490 500
THPEVLSQKP FDKVIIQSQL PIGTLKHNMT YFCKTHNSVG NSSQYFRAVS
510 520 530 540 550
LGQSKQLPDE SLFTPVVVAC MSVMSLLVLL LLLLLYKYKQ KPKYQVRWKI
560 570 580 590 600
IERYEGNSYT FIDPTQLPYN EKWEFPRNNL QFGKTLGAGA FGKVVEATAF
610 620 630 640 650
GLGKEDAVLK VAVKMLKSTA HADEKEALMS ELKIMSHLGQ HENIVNLLGA
660 670 680 690 700
CTHGGPVLVI TEYCCYGDLL NFLRRKAEAM LGPSLSPGQD SEGDSSYKNI
710 720 730 740 750
HLEKKYVRRD SGFSSQGVDT YVEMRPVSTS SSDSFFKQDL DKEASRPLEL
760 770 780 790 800
WDLLHFSSQV AQGMAFLASK NCIHRDVAAR NVLLTSGHVA KIGDFGLARD
810 820 830 840 850
IMNDSNYVVK GNARLPVKWM APESIFDCVY TVQSDVWSYG ILLWEIFSLG
860 870 880 890 900
LNPYPGILVN NKFYKLVKDG YQMAQPVFAP KNIYSIMQSC WDLEPTRRPT
910 920 930 940 950
FQQICFLLQE QARLERRDQD YANLPSSGGS SGSDSGGGSS GGSSSEPEEE
960 970 977
SSSEHLACCE PGDIAQPLLQ PNNYQFC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3547 Collapse assay details

Data source: Panorama

Official Gene Symbol
Csf1r
Peptide Sequence
LEIPLNSDFQDNYYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
244
Peptide End
258
CPTAC ID
non-CPTAC-3547
Peptide Molecular Mass
1,857.8785
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 14.7 5.3 3.9 15.6 7 6.9 21.4 8.8 7.9 15 15 15
b2 (1+) 5 6.6 5.3 14.3 8.2 5.9 15.1 10.5 7.9 15 15 15
y12 (2+) 6.3 3.4 5.7 17.9 5.8 8.1 19 6.7 9.9 15 15 15
y13 (2+) 9 6.9 6.3 13.3 7 8.6 16.1 9.8 10.7 15 15 15
y2 (1+) 19.5 8.9 9.4 21.7 13.2 15.4 29.2 15.9 18 15 15 15
sum 5 2.7 3.5 14.7 4.8 6.2 15.5 5.5 7.1 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3548 Collapse assay details

Data source: Panorama

Official Gene Symbol
Csf1r
Peptide Sequence
VIIQSQLPIGTLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
464
Peptide End
476
CPTAC ID
non-CPTAC-3548
Peptide Molecular Mass
1,408.8654
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.9 3.1 3.5 15.2 4.5 9.1 16.3 5.5 9.7 15 15 15
y11 (2+) 7.5 6.1 4.3 17.8 8.1 6.1 19.3 10.1 7.5 15 15 15
y6 (1+) 4.1 4.7 2.9 13.8 5.2 8.5 14.4 7 9 15 15 15
y7 (1+) 6.9 5.6 7.8 12.6 6.3 9.8 14.4 8.4 12.5 15 15 15
y9 (1+) 5.2 5 4.9 14.4 7.5 8.8 15.3 9 10.1 15 15 15
sum 2.5 3 2.1 14.1 5.2 7.3 14.3 6 7.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3055 Collapse assay details

Data source: Panorama

Official Gene Symbol
CSF1R
Peptide Sequence
NNLQFGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
580
Peptide End
586
CPTAC ID
CPTAC-3055
Peptide Molecular Mass
819.4239
Species
Homo Sapiens
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 21.8 5.4 12.9 25.6 16.4 16.6 33.6 17.3 21 15 15 15
y5 (1+) 6.4 20 3.3 10.9 31.5 5.1 12.6 37.3 6.1 14 15 14
y3 (1+) 42.3 5.2 9.5 79.9 9.7 12.9 90.4 11 16 15 15 15
sum 37.2 5.6 8.9 74.4 10.7 12.4 83.2 12.1 15.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2838 Collapse assay details

Data source: Panorama

Official Gene Symbol
CSF1R
Peptide Sequence
VVEATAFGLGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
596
Peptide End
606
CPTAC ID
CPTAC-2838
Peptide Molecular Mass
1,090.6023
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 18 11.5 5.9 17.9 14.1 9.9 25.4 18.2 11.5 15 15 15
y5 (1+) 10.6 12.6 6 14.7 15.5 9.6 18.1 20 11.3 15 15 15
y4 (1+) 18.6 9.6 5.2 21.9 13.5 9.3 28.7 16.6 10.7 15 15 15
sum 13.9 10.9 5.3 14.8 14.1 9.2 20.3 17.8 10.6 15 15 15


Additional Resources and Comments