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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Jchain Igj
Sequence Length (AA) Molecular Weight (Da)
159 18014
Protein Name
Immunoglobulin J chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKTHLLLWGV LAIFVKAVLV TGDDEATILA DNKCMCTRVT SRIIPSTEDP
60 70 80 90 100
NEDIVERNIR IVVPLNNREN ISDPTSPLRR NFVYHLSDVC KKCDPVEVEL
110 120 130 140 150
EDQVVTATQS NICNEDDGVP ETCYMYDRNK CYTTMVPLRY HGETKMVQAA
159
LTPDSCYPD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-659 Collapse assay details

Data source: Panorama

Official Gene Symbol
JCHAIN
Peptide Modified Sequence
EN[+1.0]ISDPTSPLR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
71
Peptide - Site of Modification
2
Peptide Start
70
Peptide End
80
CPTAC ID
CPTAC-659
Peptide Molecular Mass
1,228.5935
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 6.7 6.9 6.5 8.7 6.9 6.8 11 9.8 9.4 15 15 15
y7 (1+) 9.8 7.2 8.9 17.8 7.1 9.9 20.3 10.1 13.3 15 15 15
y6 (1+) 6.3 5.2 6.5 8.4 5 6.2 10.5 7.2 9 15 15 15
sum 5 5.5 6.6 7.5 5.2 6.4 9 7.6 9.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3651 Collapse assay details

Data source: Panorama

Official Gene Symbol
Jchain
Peptide Sequence
IIPSTEDPNEDIVER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
43
Peptide End
57
CPTAC ID
non-CPTAC-3651
Peptide Molecular Mass
1,725.8421
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 8.6 5.1 7.1 15.6 6 8.8 17.8 7.9 11.3 15 15 15
b2 (1+) 5.3 3.9 4.8 13.8 6.4 5.5 14.8 7.5 7.3 15 15 15
y13 (2+) 4.3 5.5 5 15 6 7.2 15.6 8.1 8.8 15 15 15
y4 (1+) 7.5 3.2 6.7 12.3 3.3 8.7 14.4 4.6 11 15 15 15
y8 (1+) 4.4 5.5 6.4 12.4 7 7.3 13.2 8.9 9.7 15 15 15
sum 3.4 3.4 3.7 13.6 4.6 6.6 14 5.7 7.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3920 Collapse assay details

Data source: Panorama

Official Gene Symbol
Jchain
Peptide Sequence
IVVPLNNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
61
Peptide End
68
CPTAC ID
non-CPTAC-3920
Peptide Molecular Mass
923.5553
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 3.9 2.7 3.3 7.3 10.5 10.3 8.3 10.8 10.8 15 15 15
y5 (2+) 5.6 1.6 3.5 6.5 9.1 8.4 8.6 9.2 9.1 15 15 15
y6 (2+) 6.1 3.1 2.5 6.3 11.2 12.4 8.8 11.6 12.6 15 15 15
y5 (1+) 3.9 3.1 3.6 7.5 11.6 9.9 8.5 12 10.5 15 15 15
y6 (1+) 8.7 5.8 3.6 7.7 11.6 12.3 11.6 13 12.8 15 15 15
sum 2 1.6 1.9 5.9 10.4 9.9 6.2 10.5 10.1 15 15 15


Additional Resources and Comments