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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Pigr

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Pigr N/A
Sequence Length (AA) Molecular Weight (Da)
771 84999
Protein Name
Polymeric immunoglobulin receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLYLFTLLV TVFSGVSTKS PIFGPQEVSS IEGDSVSITC YYPDTSVNRH
60 70 80 90 100
TRKYWCRQGA SGMCTTLISS NGYLSKEYSG RANLINFPEN NTFVINIEQL
110 120 130 140 150
TQDDTGSYKC GLGTSNRGLS FDVSLEVSQV PELPSDTHVY TKDIGRNVTI
160 170 180 190 200
ECPFKRENAP SKKSLCKKTN QSCELVIDST EKVNPSYIGR AKLFMKGTDL
210 220 230 240 250
TVFYVNISHL THNDAGLYIC QAGEGPSADK KNVDLQVLAP EPELLYKDLR
260 270 280 290 300
SSVTFECDLG REVANEAKYL CRMNKETCDV IINTLGKRDP DFEGRILITP
310 320 330 340 350
KDDNGRFSVL ITGLRKEDAG HYQCGAHSSG LPQEGWPIQT WQLFVNEEST
360 370 380 390 400
IPNRRSVVKG VTGGSVAIAC PYNPKESSSL KYWCRWEGDG NGHCPVLVGT
410 420 430 440 450
QAQVQEEYEG RLALFDQPGN GTYTVILNQL TTEDAGFYWC LTNGDSRWRT
460 470 480 490 500
TIELQVAEAT REPNLEVTPQ NATAVLGETF TVSCHYPCKF YSQEKYWCKW
510 520 530 540 550
SNKGCHILPS HDEGARQSSV SCDQSSQLVS MTLNPVSKED EGWYWCGVKQ
560 570 580 590 600
GQTYGETTAI YIAVEERTRG SSHVNPTDAN ARAKVALEEE VVDSSISEKE
610 620 630 640 650
NKAIPNPGPF ANEREIQNVG DQAQENRASG DAGSADGQSR SSSSKVLFST
660 670 680 690 700
LVPLGLVLAV GAIAVWVARV RHRKNVDRMS ISSYRTDISM ADFKNSRDLG
710 720 730 740 750
GNDNMGASPD TQQTVIEGKD EIVTTTECTA EPEESKKAKR SSKEEADMAY
760 770 771
SAFLLQSSTI AAQVHDGPQE A

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-685 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIGR
Peptide Modified Sequence
VPGN[+1.0]VTAVLGETLK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
469
Peptide - Site of Modification
4
Peptide Start
466
Peptide End
479
CPTAC ID
CPTAC-685
Peptide Molecular Mass
1,397.7766
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 7.8 5.1 4.9 7.7 5.8 5.4 11 7.7 7.3 15 15 15
y9 (1+) 3.4 4.9 3.4 3.9 4.8 4.9 5.2 6.9 6 15 15 15
y8 (1+) 4.6 4.7 3 6.6 4.8 4.8 8 6.7 5.7 15 15 15
sum 4.1 4.6 2.9 4.5 4.4 4.3 6.1 6.4 5.2 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3738 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pigr
Peptide Sequence
VALEEEVVDSSISEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
585
Peptide End
599
CPTAC ID
non-CPTAC-3738
Peptide Molecular Mass
1,632.8094
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 17 11.4 6.9 16 12.6 8.4 23.3 17 10.9 15 15 15
y3 (1+) 12.7 6.7 4.3 13.5 7.8 5 18.5 10.3 6.6 15 15 15
y7 (1+) 17.5 6.5 4 15.9 8.1 8.8 23.6 10.4 9.7 15 15 15
y8 (1+) 10.4 5.2 6.5 11.3 6.3 5.7 15.4 8.2 8.6 15 15 15
y9 (1+) 13.6 8.1 8.5 12.1 10.3 10 18.2 13.1 13.1 15 15 15
sum 9.1 2.3 1.8 7.2 4.4 4.1 11.6 5 4.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments