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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Postn Osf2
Sequence Length (AA) Molecular Weight (Da)
838 93144
Protein Name
Periostin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVPLLPLYAL LLLFLCDINP ANANSYYDKV LAHSRIRGRD QGPNVCALQQ
60 70 80 90 100
ILGTKKKYFS SCKNWYQGAI CGKKTTVLYE CCPGYMRMEG MKGCPAVMPI
110 120 130 140 150
DHVYGTLGIV GATTTQHYSD VSKLREEIEG KGSYTYFAPS NEAWENLDSD
160 170 180 190 200
IRRGLENNVN VELLNALHSH MVNKRMLTKD LKHGMVIPSM YNNLGLFINH
210 220 230 240 250
YPNGVVTVNC ARVIHGNQIA TNGVVHVIDR VLTQIGTSIQ DFLEAEDDLS
260 270 280 290 300
SFRAAAITSD LLESLGRDGH FTLFAPTNEA FEKLPRGVLE RIMGDKVASE
310 320 330 340 350
ALMKYHILNT LQCSEAITGG AVFETMEGNT IEIGCEGDSI SINGIKMVNK
360 370 380 390 400
KDIVTKNGVI HLIDEVLIPD SAKQVIELAG KQQTTFTDLV AQLGLASSLK
410 420 430 440 450
PDGEYTLLAP VNNAFSDDTL SMDQRLLKLI LQNHILKVKV GLSDLYNGQI
460 470 480 490 500
LETIGGKQLR VFVYRTAICI ENSCMVRGSK QGRNGAIHIF REIIQPAEKS
510 520 530 540 550
LHDKLRQDKR FSIFLSLLEA ADLKDLLTQP GDWTLFAPTN DAFKGMTSEE
560 570 580 590 600
RELLIGDKNA LQNIILYHLT PGVYIGKGFE PGVTNILKTT QGSKIYLKGV
610 620 630 640 650
NETLLVNELK SKESDIMTTN GVIHVVDKLL YPADIPVGND QLLELLNKLI
660 670 680 690 700
KYIQIKFVRG STFKEIPMTV YTTKIITKVV EPKIKVIQGS LQPIIKTEGP
710 720 730 740 750
AMTKIQIEGD PDFRLIKEGE TVTEVIHGEP VIKKYTKIID GVPVEITEKQ
760 770 780 790 800
TREERIITGP EIKYTRISTG GGETGETLQK FLQKEVSKVT KFIEGGDGHL
810 820 830 838
FEDEEIKRLL QGDTPAKKIP ANKRVQGPRR RSREGRSQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3739 Collapse assay details

Data source: Panorama

Official Gene Symbol
Postn
Peptide Sequence
LLYPADIPVGNDQLLELLNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
629
Peptide End
648
CPTAC ID
non-CPTAC-3739
Peptide Molecular Mass
2,237.2307
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 2.8 2.5 1.8 9.7 4.1 6 10.1 4.8 6.3 15 15 15
y17 (2+) 4.6 5.8 4.9 6.4 8.6 10.9 7.9 10.4 12 15 15 15
y6 (1+) 65.8 28.4 46.6 66 39.2 41.6 93.2 48.4 62.5 15 15 15
b7 (1+) 3.9 2.4 2.1 9.4 4.2 3.1 10.2 4.8 3.7 15 15 15
y7 (3+) 20.8 22.3 19.9 33.4 25.8 24.3 39.3 34.1 31.4 15 15 15
sum 2.6 1.1 2.1 8.2 4.4 3.8 8.6 4.5 4.3 15 15 15


Additional Resources and Comments