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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Prtg N/A
Sequence Length (AA) Molecular Weight (Da)
1191 130533
Protein Name
Protogenin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPPVRPGML PLLLLLLLPP LGSVPGVWSF SELFFMKEPQ DATVTRKDPV
60 70 80 90 100
VLDCQAHGEG PIKVTWLKNG AKLSENKRIQ VLSNGSLYIS EVEGRRGEQS
110 120 130 140 150
DEGFYQCLAV NKYGAILSQK AHLTLSTISA FEVHPVSTEV HEGGVARFSC
160 170 180 190 200
KISSTPPAVI TWEFNRTALP TTMDRVTALP SGVLQIYDVG PEDAGNYRCV
210 220 230 240 250
AATIAHKRKS MEASLTIVAA NETRSFYMPT IIASPQNVTA SLHQTVVLEC
260 270 280 290 300
MATGYPRPII SWSRLDHKSI DVFNTRVLGN GNLIISDVKL QHAGVYVCRA
310 320 330 340 350
TTPGTRNFTV AMATLTVLAP PSFVEWPESL TRPRAGTARF VCQAEGIPSP
360 370 380 390 400
KMSWLKNGRR IHSNGRIKMY NSKLVINQII PEDDAIYQCM AENSQGSVLS
410 420 430 440 450
RARLTVVMSE DRPSAPYNVH AETMSSSAIL LAWERPLYNS DKVIAYSVHY
460 470 480 490 500
MKAEGLNNEE YQVVLGNDTT HYIIDDLEPD SNYTFYIVAY MPLGASQMSD
510 520 530 540 550
HVTQNTLEDV PLRPPEISLT SRSPTDILVS WLPIPAKYRR GQVVLYRLSF
560 570 580 590 600
RLSTENAIQV VELPGTVHEY LLEGLKPDSV YLVRITAATR VGLGESSVWT
610 620 630 640 650
SHRTPKATSV KAPKSPELHL EPLNCTTISV RWLQDTEDPA AIRGYKLFYK
660 670 680 690 700
EEGQQEHGPI FLDTGDLLYT LSGLDPRRKY HVRLLAYNNM EEGYQADQTV
710 720 730 740 750
STPGCVSVRD RMVPPPPPPH HLYAKANTSS SIFLHWRRPA FTTAQVINYT
760 770 780 790 800
IRCNPVGLQN ASLVLYLQTS ETHMLVQGLE PNTKYEFAVR LHVDQLSSPW
810 820 830 840 850
SPVVYHSTLP EAPTGPPVGV KVTLIEDDTA LVSWKPPDGP ETVVTRYTIL
860 870 880 890 900
YASRKAWIAG EWQVLHREGA ITMALLENLV AGNVYIVKIS ASNEVGEGPF
910 920 930 940 950
SNSVELAVLP KDASESNQRP KRLDSSNAKV YSGYYHLDQK SMTGIAVGVG
960 970 980 990 1000
IALTCILICV LILIYRSKAR KSSASKTAQS GTQPLSQASA SVAAGSDMGK
1010 1020 1030 1040 1050
NLERATETAE SLVPMMPSSF IDAKGGTDLI INSYGPIIKN NTKKKWLFFQ
1060 1070 1080 1090 1100
DTKKIKVEQT QRRFTQAVCF YQPGTTVLIS DEDSPGSPGQ TASFPRPFGA
1110 1120 1130 1140 1150
TALDTEHSAN SEGSHETGDS GRFSHESNDE IHLSSVISST PPTSNPLAGG
1160 1170 1180 1190 1191
DSDGDAAPKK HGDPAQPLPA EQTSAPQTSA GLRYAAEGFP V

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3740 Collapse assay details

Data source: Panorama

Official Gene Symbol
Prtg
Peptide Sequence
IQVLSNGSLYISEVEGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
79
Peptide End
95
CPTAC ID
non-CPTAC-3740
Peptide Molecular Mass
1,862.9738
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 9.3 4.2 6.7 10.2 5.6 7.9 13.8 7 10.4 15 15 15
y6 (1+) 7.2 2.1 1.5 8.4 4.9 3.2 11.1 5.3 3.5 15 15 15
b11 (2+) 8.3 2.8 2.3 10.5 3.6 5.7 13.4 4.6 6.1 15 15 15
y9 (2+) 8.8 7.9 5.3 6.8 9.8 7.8 11.1 12.6 9.4 15 15 15
y2 (1+) 4.4 4.1 2.5 8.7 4.9 4 9.7 6.4 4.7 15 15 15
sum 5.5 1.2 1.5 8.2 4.1 4.5 9.9 4.3 4.7 15 15 15


Additional Resources and Comments