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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Tpm3 N/A
Sequence Length (AA) Molecular Weight (Da)
285
Protein Name
Tropomyosin alpha-3 chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGTTTIEAV KRKIQVLQQQ ADDAEERAER LQREVEGERR AREQAEAEVA
60 70 80 90 100
SLNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRALK
110 120 130 140 150
DEEKMELQEI QLKEAKHIAE EADRKYEEVA RKLVIIEGDL ERTEERAELA
160 170 180 190 200
ESRCREMDEQ IRLMDQNLKC LSAAEEKYSQ KEDKYEEEIK ILTDKLKEAE
210 220 230 240 248
TRAEFAERSV AKLEKTIDDL EDKLKCTKEE HLCTQRMLDQ TLLDLNEM

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3756 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tpm3
Peptide Sequence
EQAEAEVASLNR
Modification Type
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
43
Peptide End
54
CPTAC ID
non-CPTAC-3756
Peptide Molecular Mass
1,315.6368
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 10.3 5.8 5.5 10.2 7.3 7.3 14.5 9.3 9.1 15 15 15
b2 (1+) 11.4 6.8 3.8 18 8.4 6.6 21.3 10.8 7.6 15 15 15
y7 (1+) 13.6 4.1 5 18.6 5.8 5.2 23 7.1 7.2 15 15 15
y8 (1+) 6.5 5 4 13.5 5.7 8 15 7.6 8.9 15 15 15
y9 (1+) 15.1 8.9 5.1 20.2 8.5 5.9 25.2 12.3 7.8 15 15 15
sum 6.2 2.4 1.9 11.9 5.3 5.6 13.4 5.8 5.9 15 15 15

Additional Resources and Comments