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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Capza2 Cappa2
Sequence Length (AA) Molecular Weight (Da)
286 32967
Protein Name
F-actin-capping protein subunit alpha-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MADLEEQLSD EEKVRIAAKF IIHAPPGEFN EVFNDVRLLL NNDNLLREGA
60 70 80 90 100
AHAFAQYNLD QFTPVKIEGY EDQVLITEHG DLGNGKFLDP KNRICFKFDH
110 120 130 140 150
LRKEATDPRP YEAENAIESW RTSVETALRA YVKEHYPNGV CTVYGKKVDG
160 170 180 190 200
QQTIIACIES HQFQAKNFWN GRWRSEWKFT VTPSTTQVVG ILKIQVHYYE
210 220 230 240 250
DGNVQLVSHK DIQDSLTVSN EVQTAKEFIK IVEAAENEYQ TAISENYQTM
260 270 280 286
SDTTFKALRR QLPVTRTKID WNKILSYKIG KEMQNA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3771 Collapse assay details

Data source: Panorama

Official Gene Symbol
Capza2
Peptide Sequence
EGAAHAFAQYNLDQFTPVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
48
Peptide End
66
CPTAC ID
non-CPTAC-3771
Peptide Molecular Mass
2,106.0171
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b10 (2+) 13.5 6 5.1 12.8 7.5 8.6 18.6 9.6 10 15 15 15
b9 (2+) 3.8 3.6 4.6 9.9 8.3 8.1 10.6 9 9.3 15 15 15
y9 (2+) 14.7 5.5 4.2 13.9 10 5.9 20.2 11.4 7.2 15 15 15
y3 (1+) 2.9 2.5 3.6 9.1 7.5 5.3 9.6 7.9 6.4 15 15 15
y7 (1+) 5.3 2.5 3.7 9.1 7.3 5.9 10.5 7.7 7 15 15 15
sum 2.6 0.9 2.8 7.5 7 5.3 7.9 7.1 6 15 15 15


Additional Resources and Comments