Eno3, CPTAC-3791 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Eno3	Eno-3

Sequence Length (AA)	Molecular Weight (Da)
434	47025

Protein Name
Beta-enolase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAMQKIFARE	ILDSRGNPTV	EVDLHTAKGR	FRAAVPSGAS	TGIYEALELR
60	70	80	90	100
DGDKARYLGK	GVLKAVEHIN	KTLGPALLEK	KLSVVDQEKV	DKFMIELDGT
110	120	130	140	150
ENKSKFGANA	ILGVSLAVCK	AGAAEKGVPL	YRHIADLAGN	PDLVLPVPAF
160	170	180	190	200
NVINGGSHAG	NKLAMQEFMI	LPVGASSFKE	AMRIGAEVYH	HLKGVIKAKY
210	220	230	240	250
GKDATNVGDE	GGFAPNILEN	NEALELLKTA	IQAAGYPDKV	VIGMDVAASE
260	270	280	290	300
FYRNGKYDLD	FKSPDDPARH	ISGEKLGELY	KNFIQNYPVV	SIEDPFDQDD
310	320	330	340	350
WATWTSFLSG	VDIQIVGDDL	TVTNPKRIAQ	AVEKKACNCL	LLKVNQIGSV
360	370	380	390	400
TESIQACKLA	QSNGWGVMVS	HRSGETEDTF	IADLVVGLCT	GQIKTGAPCR
410	420	430	434
SERLAKYNQL	MRIEEALGDK	AVFAGRKFRN	PKAK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3792 Collapse assay details

Data source: Panorama

Official Gene Symbol: Eno3
Peptide Sequence: GNPTVEVDLHTAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 16
Peptide End: 28

CPTAC ID: non-CPTAC-3792
Peptide Molecular Mass: 1,379.7045
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b8 (2+)	100.3	69.7	89.2	141.8	120.5	115.8	173.7	139.2	146.2	15	15	15
b8 (1+)	162.4	131.5	89.8	193.1	170	143.5	252.3	214.9	169.3	15	15	15
y11 (3+)	15.8	5.6	4.5	16.1	9.5	6.3	22.6	11	7.7	15	15	15
y11 (2+)	13.7	7.5	3.1	14.4	12.4	6.2	19.9	14.5	6.9	15	15	15
y8 (1+)	46.7	8	10	58.2	11.6	9.9	74.6	14.1	14.1	15	15	15
sum	7.7	4.5	2.7	7.7	9.8	5.3	10.9	10.8	5.9	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3791 Collapse assay details

Data source: Panorama

Official Gene Symbol: Eno3
Peptide Sequence: IEEALGDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 413
Peptide End: 420

CPTAC ID: non-CPTAC-3791
Peptide Molecular Mass: 873.4444
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.