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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

H2afy2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
H2afy2 H2afy3
Sequence Length (AA) Molecular Weight (Da)
372 40092
Protein Name
Core histone macro-H2A.2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSGRSGKKKM SKLSRSARAG VIFPVGRLMR YLKKGTFKYR ISVGAPVYMA
60 70 80 90 100
AVIEYLAAEI LELAGNAARD NKKARIAPRH ILLAVANDEE LNQLLKGVTI
110 120 130 140 150
ASGGVLPRIH PELLAKKRGT KGKSETILSP PPEKRGRKAA SGKKGGKKSK
160 170 180 190 200
ATKPRTSKKS KAKDSDKEGT SNSTSEDGPG DGFTILSSKS LVLGQKLSLT
210 220 230 240 250
QSDISHIGSM RVEGIVHPTT AEIDLKEEIG KALEKAGGKE FLETVKELRK
260 270 280 290 300
SQGPLEVAEA AVSQSSGLAA KFVIHCHIPQ WGSDKCEEQL EETIKNCLSA
310 320 330 340 350
AEDKKLKSVA FPPFPSGRNC FPKQTAAQVT LKAISAHFDD SSSSSLKNVY
360 370 372
FLLFDSESIG IYVQEMAKLD TK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3795 Collapse assay details

Data source: Panorama

Official Gene Symbol
H2afy2
Peptide Sequence
GVTIASGGVLPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
97
Peptide End
108
CPTAC ID
non-CPTAC-3795
Peptide Molecular Mass
1,125.6506
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 10.6 8.2 6.9 12.1 11 13.6 16.1 13.7 15.3 15 15 15
y7 (1+) 12.7 7.8 6 15.2 9.5 10 19.8 12.3 11.7 15 15 15
y6 (1+) 14.3 10.9 7.3 18.8 14.3 11.3 23.6 18 13.5 15 15 15
y2 (1+) 14.5 7.1 4.9 14.7 8.5 5.8 20.6 11.1 7.6 15 15 15
b3 (1+) 20.3 6.8 8.1 25.1 9 9.3 32.3 11.3 12.3 15 15 15
sum 7.4 4.2 2.4 10.8 7.3 8.4 13.1 8.4 8.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments