Glod4, CPTAC-3812 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Glod4	N/A

Sequence Length (AA)	Molecular Weight (Da)
298	33317

Protein Name
Glyoxalase domain-containing protein 4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MATRRALHFV	FKVKNRFQTV	HFFRDVLGMQ	VLRHEEFEEG	CKAACNGPYD
60	70	80	90	100
GKWSKTMVGF	GPEDDHFVAE	LTYNYGIGDY	KLGNDFMGIT	LASSQAVSNA
110	120	130	140	150
RKLEWPLSKV	AEGIFETEAP	GGYKFYLQDR	SPSQSDPVLK	VTLAVSDLQK
160	170	180	190	200
SLNYWSNLLG	MKIYEQDEEK	QRALLGYADN	QCKLELQGIQ	GAVDHAAAFG
210	220	230	240	250
RIAFSCPQKE	LPDLEDLMKR	ESHSILTPLV	SLDTPGKATV	QVVILADPDG
260	270	280	290	298
HEICFVGDEA	FRELSKMDPK	GSKLLDDAME	ADKSDEWFAT	RNKPKASG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3812 Collapse assay details

Data source: Panorama

Official Gene Symbol: Glod4
Peptide Sequence: LELQGIQGAVDHAAAFGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 184
Peptide End: 201

CPTAC ID: non-CPTAC-3812
Peptide Molecular Mass: 1,851.9591
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b16 (3+)	14.9	15.4	6.6	17.5	13.8	7	23	20.7	9.6	15	15	15
y16 (3+)	8.5	5.5	4.9	15.8	9.5	4.4	17.9	11	6.6	15	15	15
y14 (2+)	12.9	6.7	4.6	15	6.8	8.8	19.8	9.5	9.9	15	15	15
y15 (2+)	22	9.3	4.8	37.2	12	7.2	43.2	15.2	8.7	15	15	15
y16 (2+)	18.1	6.7	2.2	23.1	9.8	7.2	29.3	11.9	7.5	15	15	15
sum	7	2.6	1.9	15.6	6.9	4.4	17.1	7.4	4.8	15	15	15

Glod4

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3812 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Glod4

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3812 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments