Hmgb2, CPTAC-3820 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Hmgb2	Hmg2

Sequence Length (AA)	Molecular Weight (Da)
210	24162

Protein Name
High mobility group protein B2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGKGDPNKPR	GKMSSYAFFV	QTCREEHKKK	HPDSSVNFAE	FSKKCSERWK
60	70	80	90	100
TMSAKEKSKF	EDLAKSDKAR	YDREMKNYVP	PKGDKKGKKK	DPNAPKRPPS
110	120	130	140	150
AFFLFCSENR	PKIKIEHPGL	SIGDTAKKLG	EMWSEQSAKD	KQPYEQKAAK
160	170	180	190	200
LKEKYEKDIA	AYRAKGKSEA	GKKGPGRPTG	SKKKNEPEDE	EEEEEEEEEE
210
DDEEEEEDEE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3820 Collapse assay details

Data source: Panorama

Official Gene Symbol: Hmgb2
Peptide Sequence: IEHPGLSIGDTAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 115
Peptide End: 127

CPTAC ID: non-CPTAC-3820
Peptide Molecular Mass: 1,336.6987
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	5.3	3	3.3	6	7.2	4.5	8	7.8	5.6	15	15	15
y3 (1+)	24.9	5.2	5.4	19.9	7.5	7.7	31.9	9.1	9.4	15	15	15
b3 (1+)	14.5	7.5	7.6	37.2	43.5	43.7	39.9	44.1	44.4	15	15	15
b7 (1+)	11.2	9.6	4.1	11	10.5	5.1	15.7	14.2	6.5	15	15	15
b7 (2+)	17.1	9.8	4.9	15.7	10.3	7.5	23.2	14.2	9	15	15	15
sum	4.6	3.2	2.9	12.3	15.8	13.8	13.1	16.1	14.1	15	15	15

Hmgb2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3820 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Hmgb2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3820 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments