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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Itga2 N/A
Sequence Length (AA) Molecular Weight (Da)
1178 128955
Protein Name
Integrin alpha-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGPGQAGGAL LLRLLMLVQG ILNCLAYNVG LPGAKIFSGP SSEQFGYSVQ
60 70 80 90 100
QLTNPQGNWL LVGSPWSGFP ENRMGDVYKC PVDLPTATCE KLNLQNSASI
110 120 130 140 150
SNVTEIKTNM SLGLTLTRNP GTGGFLTCGP LWAHQCGNQY YATGICSDVS
160 170 180 190 200
PDFQFLTSFS PAVQACPSLV DVVVVCDESN SIYPWEAVKN FLVKFVTGLD
210 220 230 240 250
IGPKKTQVAL IQYANEPRII FNLNDFETKE DMVQATSETR QHGGDLTNTF
260 270 280 290 300
RAIEFARDYA YSQTSGGRPG ATKVMVVVTD GESHDGSKLK TVIQQCNDDE
310 320 330 340 350
ILRFGIAVLG YLNRNALDTK NLIKEIKAIA STPTERYFFN VADEAALLEK
360 370 380 390 400
AGTLGEQIFS IEGTVQGGDN FQMEMAQVGF SADYAPQNDI LMLGAVGAFD
410 420 430 440 450
WSGTLVQETS HKPVIFPKQA FDQVLQDRNH SSFLGYSVAA ISTEDGVHFV
460 470 480 490 500
AGAPRANYTG QIVLYSVNKQ GNVTVIQSHR GDQIGSYFGS VLCSVDVDKD
510 520 530 540 550
TITDVLLVGA PTYMNDLKKE EGKVYLFTIT KGILNQHQFL EGPEGTGNAR
560 570 580 590 600
FGSAIAALSD INMDGFNDVI VGSPVENENS GAVYIYNGHQ GTIRTKYSQK
610 620 630 640 650
ILGSNGAFRR HLQFFGRSLD GYGDLNGDSI TDVSIGALGQ VIQLWSQSIA
660 670 680 690 700
DVAIEALFTP DKITLLNKDA KITLKLCFRA EFRPAGQNNQ VAILFNMTLD
710 720 730 740 750
ADGHSSRVTS RGVFRENSER FLQKNMVVNE VQKCSEHHIS IQKPSDVVNP
760 770 780 790 800
LDLRVDISLE NPGTSPALEA YSETVKVFSI PFYKECGSDG ICISDLILDV
810 820 830 840 850
QQLPAIQTQS FIVSNQNKRL TFSVILKNRG ESAYNTVVLA EFSENLFFAS
860 870 880 890 900
FSMPVDGTEV TCEVGSSQKS VTCDVGYPAL KSEQQVTFTI NFDFNLQNLQ
910 920 930 940 950
NQAAINFQAF SESQETNKAD NSVSLTIPLL YDAELHLTRS TNINFYEISS
960 970 980 990 1000
DENAPSVIKS VEDIGPKFIF SLKVTAGSAP VSMALVTIHI PQYTKEKNPL
1010 1020 1030 1040 1050
LYLTGIQTDQ AGDISCTAEI NPLKLPHTAP SVSFKNENFR HTKELDCRTT
1060 1070 1080 1090 1100
SCSNITCWLK DLHMKAEYFI NVTTRVWNRT FAASTFQTVQ LTAAAEIDTH
1110 1120 1130 1140 1150
NPQLFVIEEN AVTIPLMIMK PTEKAEVPTG VIIGSIIAGI LLLLAMTAGL
1160 1170 1178
WKLGFFKRQY KKMGQNPDEM DETTELNS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3831 Collapse assay details

Data source: Panorama

Official Gene Symbol
Itga2
Peptide Sequence
FVTGLDIGPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
195
Peptide End
204
CPTAC ID
non-CPTAC-3831
Peptide Molecular Mass
1,045.5808
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 2.3 1.7 2.8 8.7 7.7 4.7 9 7.9 5.5 15 15 15
y4 (1+) 4.1 3 2.8 9.2 7.8 4.7 10.1 8.4 5.5 15 15 15
y3 (1+) 4 2.7 2.2 9.2 8.4 6.4 10 8.8 6.8 15 15 15
b2 (1+) 2.4 1.9 2.1 8.3 8.4 4.8 8.6 8.6 5.2 15 15 15
b5 (1+) 18.6 22.4 25.4 38.5 23.5 25.4 42.8 32.5 35.9 15 15 15
sum 1.4 1 1.3 8.5 7.7 4.3 8.6 7.8 4.5 15 15 15


Additional Resources and Comments