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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Krt14 Krt1-14
Sequence Length (AA) Molecular Weight (Da)
484 52867
Protein Name
Keratin, type I cytoskeletal 14
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MATCSRQFTS SSSMKGSCGI GGGSSRMSSI LAGGSCRAPS TYGGMSVTSS
60 70 80 90 100
RFSSGGACGI GGGYGGSFSS SSFGGGLGSG FGGRFDGFGG GFGGGLGGGF
110 120 130 140 150
GGGLGGGLGG GIGDGLLVGS EKVTMQNLND RLATYLDKVR ALEEANTELE
160 170 180 190 200
VKIRDWYQRQ RPTEIKDYSP YFKTIEDLKS KILAATVDNA NVLLQIDNAR
210 220 230 240 250
LAADDFRTKF ETEQSLRMSV EADINGLRRV LDELTLARAD LEMQIESLKE
260 270 280 290 300
ELAYLKKNHE EEMASMRGQV GGDVNVEMDA APGVDLSRIL NEMRDQYEKM
310 320 330 340 350
AEKNRKDAEE WFFSKTEELN REVATNSELV QSGKSEISEL RRTMQNLEIE
360 370 380 390 400
LQSQLSMKAS LENNLEETKG RYCMQLAQIQ EMIGSVEEQL AQLRCEMEQQ
410 420 430 440 450
NQEYKILLDV KTRLEQEIAT YRRLLEGEDA HLSSSQFSSS SQFSSGSQSS
460 470 480 484
RDVTSTNRQI RTKVMDVHDG KVVSTHEQVL RTKN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3833 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt14
Peptide Sequence
ASLENNLEETK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
359
Peptide End
369
CPTAC ID
non-CPTAC-3833
Peptide Molecular Mass
1,246.6041
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.6 6.4 3.6 9.7 9.7 6.7 12.3 11.6 7.6 15 15 15
y7 (1+) 5.6 6.2 6 8.6 11.7 6.4 10.3 13.2 8.8 15 15 15
y4 (1+) 15.7 9.4 7 14.8 14 10.1 21.6 16.9 12.3 15 15 15
y2 (1+) 20.4 5 4.6 20.2 6.9 5.6 28.7 8.5 7.2 15 15 15
y9 (2+) 13.1 8 4.7 13.2 10 5.6 18.6 12.8 7.3 15 15 15
sum 5.2 2.9 2.6 7.3 8.5 3.9 9 9 4.7 15 15 15


Additional Resources and Comments