Ranbp1, CPTAC-3859 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Ranbp1	Htf9-a, Htf9a

Sequence Length (AA)	Molecular Weight (Da)
203	23596

Protein Name
Ran-specific GTPase-activating protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAAKDSHED	HDTSTENADE	SNHDPQFEPI	VSLPEQEIKT	LEEDEEELFK
60	70	80	90	100
MRAKLFRFAS	ENDLPEWKER	GTGDVKLLKH	KEKGTIRLLM	RRDKTLKICA
110	120	130	140	150
NHYITPMMEL	KPNAGSDRAW	VWNTHADFAD	ECPKPELLAI	RFLNAENAQK
160	170	180	190	200
FKTKFEECRK	EIEEREKKGP	GKNDNAEKVA	EKLEALSVRE	AREEAEEKSE
203
EKQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3860 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ranbp1
Peptide Sequence: LEALSVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 183
Peptide End: 189

CPTAC ID: non-CPTAC-3860
Peptide Molecular Mass: 786.4600
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b4 (2+)	68	64.1	92.4	120.9	164.7	122.3	138.7	176.7	153.3	15	15	15
y3 (1+)	12.3	7	5.1	15.5	9.2	5.9	19.8	11.6	7.8	15	15	15
y4 (1+)	17.5	6.4	4.5	27.8	11.7	6.3	32.8	13.3	7.7	15	15	15
y5 (1+)	4.4	3.6	4.3	7.8	9.8	5.4	9	10.4	6.9	15	15	15
y6 (1+)	9.2	5.8	5.2	10.8	11.1	8.1	14.2	12.5	9.6	15	15	15
sum	5.6	2.9	2.9	7.2	9.3	4.6	9.1	9.7	5.4	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3859 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ranbp1
Peptide Sequence: TLEEDEEELFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 40
Peptide End: 50

CPTAC ID: non-CPTAC-3859
Peptide Molecular Mass: 1,380.6297
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.