Prep, CPTAC-3867 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Prep	Pep

Sequence Length (AA)	Molecular Weight (Da)
710	80752

Protein Name
Prolyl endopeptidase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLSFQYPDVY	RDETSVQEYH	GHKICDPYSW	LEDPDSEQTK	AFVEAQNKIT
60	70	80	90	100
VPFLEQCPIR	GLYKERMTEL	YDYPKYSCHF	KKGKRYFYFY	NTGLQNQRVL
110	120	130	140	150
YVQDSLEGEA	RVFLDPNTLS	DDGTVALRGY	AFSEDGEYFA	YGLSASGSDW
160	170	180	190	200
VTIKFMKVDG	AKELPDVLER	VKFTCMAWTH	DGKGMFYNSY	PQQDGKSDGT
210	220	230	240	250
ETSTNLHQKL	CYHVLGTDQS	EDILCAEFPD	EPKWMGGAEL	SDDGRYVLLS
260	270	280	290	300
IWEGCDPVNR	LWYCDLQQEP	NGITGILKWV	KLIDNFEGEY	DYVTNEGTVF
310	320	330	340	350
TFKTNRNSPN	YRLINIDFTD	PDESKWKVLV	PEHEKDVLEW	VACVRSNFLV
360	370	380	390	400
LCYLHDVKNI	LQLHDLTTGA	LLKTFPLDVG	SVVGYSGRKK	DSEIFYQFTS
410	420	430	440	450
FLSPGVIYHC	DLTKEELEPM	VFREVTVKGI	DAADYQTIQI	FYPSKDGTKI
460	470	480	490	500
PMFIVHKKGI	KLDGSHPAFL	YGYGGFNISI	TPNYSVSRLI	FVRHMGGVLA
510	520	530	540	550
VANIRGGGEY	GETWHKGGIL	ANKQNCFDDF	QCAAEYLIKE	GYTSPKRLTI
560	570	580	590	600
NGGSNGGLLV	AACANQRPDL	FGCVIAQVGV	MDMLKFHKFT	IGHAWTTDYG
610	620	630	640	650
CSDTKQHFEW	LLKYSPLHNV	KLPEADDIQY	PSMLLLTADH	DDRVVPLHSL
660	670	680	690	700
KFIATLQYIV	GRSRKQSNPL	LIHVDTKAGH	GAGKPTAKVI	EEVSDMFAFI
710
ARCLNIEWIQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3867 Collapse assay details

Data source: Panorama

Official Gene Symbol: Prep
Peptide Sequence: NILQLHDLTTGALLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 359
Peptide End: 373

CPTAC ID: non-CPTAC-3867
Peptide Molecular Mass: 1,648.9512
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	6.5	3.7	2.5	12.1	8.7	8.2	13.7	9.5	8.6	15	15	15
y6 (3+)	88.2	74.1	65.7	64.6	76.7	75.3	109.3	106.6	99.9	15	15	15
y10 (2+)	34.8	5.5	4.8	34	8.8	7.8	48.7	10.4	9.2	15	15	15
y13 (2+)	7.4	3.7	3.1	9.8	7.9	8	12.3	8.7	8.6	15	15	15
y2 (1+)	23.8	5.2	3.9	20.9	9.5	9.5	31.7	10.8	10.3	15	15	15
sum	6.2	3.2	2.6	9.4	7.8	7.9	11.3	8.4	8.3	15	15	15

Prep

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3867 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

Prep

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3867 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments