Psmd9, CPTAC-3869 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Psmd9	N/A

Sequence Length (AA)	Molecular Weight (Da)
222	24720

Protein Name
26S proteasome non-ATPase regulatory subunit 9

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSGEDVPHRA	ESSEARAAAV	SDIQDLMRRK	EEIEAEIKAN	YDVLESQKGI
60	70	80	90	100
GMNEPLVDCE	GYPRADVDLY	QVRTARHNII	CLQNDHKALM	KQVEEALHQL
110	120	130	140	150
HARDKEKQAR	DMAEAREEAM	NRRLASNSPV	LPQAFARVNS	ISPGSPASIA
160	170	180	190	200
GLQVDDEIVE	FGSVNTQNFQ	SVQNVGTVVQ	HSEGKPLNVT	VIRRGEKHQL
210	220	222
RLIPTRWAGK	GLLGCNIIPL	QR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3869 Collapse assay details

Data source: Panorama

Official Gene Symbol: Psmd9
Peptide Sequence: ADVDLYQVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 65
Peptide End: 73

CPTAC ID: non-CPTAC-3869
Peptide Molecular Mass: 1,077.5455
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	28.8	6.4	6.2	33.2	12.2	7.4	44	13.8	9.7	15	15	15
y6 (1+)	13.1	3.8	5.4	14.4	7.1	7.5	19.5	8.1	9.2	15	15	15
y5 (1+)	13.8	7.9	8.7	13.9	10.1	7.6	19.6	12.8	11.6	15	15	15
y4 (1+)	11.5	3.3	4	12.4	7.4	5	16.9	8.1	6.4	15	15	15
y3 (1+)	26.5	9.6	7.6	26.8	11	9.2	37.7	14.6	11.9	15	15	15
sum	4.5	2.1	2.4	6	6.9	4.1	7.5	7.2	4.8	15	15	15

Psmd9

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3869 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Psmd9

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3869 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments