Set, CPTAC-3874 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Set	N/A

Sequence Length (AA)	Molecular Weight (Da)
289	33378

Protein Name
Protein SET

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAPKRQSAIL	PQPKKPRPAA	APKLEDKSAS	PGLPKGEKEQ	QEAIEHIDEV
60	70	80	90	100
QNEIDRLNEQ	ASEEILKVEQ	KYNKLRQPFF	QKRSELIAKI	PNFWVTTFVN
110	120	130	140	150
HPQVSALLGE	EDEEALHYLT	RVEVTEFEDI	KSGYRIDFYF	DENPYFENKV
160	170	180	190	200
LSKEFHLNES	GDPSSKSTEI	KWKSGKDLTK	RSSQTQNKAS	RKRQHEEPES
210	220	230	240	250
FFTWFTDHSD	AGADELGEVI	KDDIWPNPLQ	YYLVPDMDDE	EGEAEDDDDD
260	270	280	289
DEEEEGLEDI	DEEGDEDEGE	EDDDEDEGEE	GEEDEGEDD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3874 Collapse assay details

Data source: Panorama

Official Gene Symbol: Set
Peptide Sequence: EQQEAIEHIDEVQNEIDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 39
Peptide End: 56

CPTAC ID: non-CPTAC-3874
Peptide Molecular Mass: 2,194.0138
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	9.7	7.3	3.3	12	9	5.8	15.4	11.6	6.7	15	14	15
y13 (2+)	58.1	14.3	21.2	51.2	20.1	18.7	77.4	24.7	28.3	15	14	15
y14 (2+)	20.2	11.8	11.8	22.6	16.2	16.4	30.3	20	20.2	15	14	15
y15 (2+)	16.2	8.8	5.8	14.4	12.3	11.6	21.7	15.1	13	15	14	15
y5 (1+)	12	8.6	7.7	10.7	9.1	9.8	16.1	12.5	12.5	15	14	15
sum	7.7	4.3	3.9	8.1	6.8	8	11.2	8	8.9	15	14	15

Set

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3874 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Set

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3874 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments