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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Srl Sar
Sequence Length (AA) Molecular Weight (Da)
910 99184
Protein Name
Sarcalumenin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKALLLLCCF LASLLLSGQA ELQVSASGGT EDVGNLLENH FSAGDASLEE
60 70 80 90 100
KERALYADAA PGDKNLLLHY PDGREAESLE QTPAGAPSTA NGQGSETEAS
110 120 130 140 150
LSNTSAAESA PPGDVEGPGE EEEGPHAAGT LPPGGIEGPE EERPQFSSGK
160 170 180 190 200
EPGQEEAGVG VPTEGAASME AEVQEGSSGV PEELQGLLGD GPMNEAAGTA
210 220 230 240 250
ESVVTQSSPP IEGKEAEGEG NTGSDHQSTE LDGTQDAIPA GDESEAREGA
260 270 280 290 300
EDKPQDYSLQ SEDTATPGPD HQPTDLHAAS DTASSGVEAE EGAEESKAEV
310 320 330 340 350
DTESGEKVED QGEPRPSLDA GSAIEANKQS EYTQARETEN DSPEGQVSGM
360 370 380 390 400
SEEGLAEASS EEEGGEEDGS ENGEVPFKEE SGEDSGDGAS SEEEGSTPGE
410 420 430 440 450
AGEPQKTPGA TGHEEEGPQL DVEDLNTGFE GSKTQDIEAE ASEERQQERG
460 470 480 490 500
NPVIAQWEEV EDASEEAPLR DRSHIDKTLM LNEDKPADDY SAVLQRLRKI
510 520 530 540 550
YHTSIKPLEQ SYKYNELRQH EITDGEITSK PMVLFLGPWS VGKSTMINYL
560 570 580 590 600
LGLEDTRYQL YTGAEPTTSE FTVLMHGPKL KTIEGIVMAA DSARSFSPLE
610 620 630 640 650
KFGQNFLEKL IGIEVPHKLL ERVTFVDTPG IIENRKQQER GYPFNDVCQW
660 670 680 690 700
FIDRADLIFV VFDPTKLDVG LELEMLFRQL KGRESQIRII LNKADNLATQ
710 720 730 740 750
MLMRVYGALF WSLAPLINVT EPPRVYVSSF WPQDYKPDTH RELFLKEEIS
760 770 780 790 800
LLEDLNQVIE NRLENKIAFI RQHAIRVRIH ALLVDRYLQT YKDKMTFFSD
810 820 830 840 850
GELVFKDIVE DPDKFYIFKT ILAKTNVSKF DLPNREAYKD FFGINPISNF
860 870 880 890 900
KLLSQQCSYM GGCFLEKIER AITQELPSLL GSIGLGKNPG APNCDKTGCG
910
ETPKNRYKKH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3877 Collapse assay details

Data source: Panorama

Official Gene Symbol
Srl
Peptide Sequence
LIGIEVPHK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
610
Peptide End
618
CPTAC ID
non-CPTAC-3877
Peptide Molecular Mass
1,004.6019
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 3.9 2.8 4.7 8.9 7.5 7.7 9.7 8 9 15 15 15
y7 (2+) 5.7 4.1 5.9 7.1 7.2 8.2 9.1 8.3 10.1 15 15 15
y8 (2+) 10.2 4.3 3.7 13.7 9.9 7.8 17.1 10.8 8.6 15 15 15
y2 (1+) 6.1 4.9 4.9 9.3 9.1 5.8 11.1 10.3 7.6 15 15 15
y3 (1+) 4.3 3.1 5 9.8 6.8 7.8 10.7 7.5 9.3 15 15 15
sum 3 2.3 3.9 5.8 6.8 6.7 6.5 7.2 7.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments