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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Acat1 N/A
Sequence Length (AA) Molecular Weight (Da)
424 44816
Protein Name
Acetyl-CoA acetyltransferase, mitochondrial
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAALVALHGV VRRPLLRGLL QEVRCLERSY ASKPTLNEVV IVSAIRTPIG
60 70 80 90 100
SFLGSLASQP ATKLGTAAIQ GAIEKAGIPK EEVKEVYMGN VIQGGEGQAP
110 120 130 140 150
TRQATLGAGL PISTPCTTVN KVCASGMKAI MMASQSLMCG HQDVMVAGGM
160 170 180 190 200
ESMSNVPYVM SRGATPYGGV KLEDLIVKDG LTDVYNKIHM GNCAENTAKK
210 220 230 240 250
MNISRQEQDT YALSSYTRSK EAWDAGKFAS EITPITISVK GKPDVVVKED
260 270 280 290 300
EEYKRVDFSK VPKLKTVFQK ENGTITAANA STLNDGAAAL VLMTAEAAQR
310 320 330 340 350
LNVKPLARIA AFADAAVDPI DFPLAPAYAV PKVLKYAGLK KEDIAMWEVN
360 370 380 390 400
EAFSVVVLAN IKMLEIDPQK VNIHGGAVSL GHPIGMSGAR IVVHMAHALK
410 420 424
PGEFGLASIC NGGGGASALL IEKL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3882 Collapse assay details

Data source: Panorama

Official Gene Symbol
Acat1
Peptide Sequence
LNVKPLAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
301
Peptide End
308
CPTAC ID
non-CPTAC-3882
Peptide Molecular Mass
909.5760
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 17.3 8.4 6.6 17.4 12.1 6.6 24.5 14.7 9.3 15 15 15
y4 (1+) 6.7 5.4 4.1 7.1 8.5 5.1 9.8 10.1 6.5 15 15 15
y7 (2+) 7.9 5.2 5 9.7 10.5 8.3 12.5 11.7 9.7 15 15 15
y6 (2+) 6.6 6.9 7.7 8.5 11.1 7.5 10.8 13.1 10.7 15 15 15
b6 (3+) 112.6 85.4 40.7 117.3 144.8 65 162.6 168.1 76.7 15 15 15
sum 3.3 3.1 2.7 4.9 8.9 5.3 5.9 9.4 5.9 15 15 15

Additional Resources and Comments