Blvrb, CPTAC-3896 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Blvrb	N/A

Sequence Length (AA)	Molecular Weight (Da)
206	22197

Protein Name
Flavin reductase (NADPH)

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTVKKIAIFG	ATGRTGLTTL	AQAVQAGYEV	TVLVRDSSRL	PSEGPQPAHV
60	70	80	90	100
VVGDVRQAAD	VDKTVAGQEA	VIVLLGTGND	LSPTTVMSEG	TRNIVTAMKA
110	120	130	140	150
HGVDKVVACT	SAFLLWDPTK	VPPRLQDVTD	DHIRMHKILQ	ESGLKYVAVM
160	170	180	190	200
PPHIGDQPLT	GAYTVTLDGR	GPSRVISKHD	LGHFMLRCLT	TNEYDGHTTY
206
PSHQYD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3896 Collapse assay details

Data source: Panorama

Official Gene Symbol: Blvrb
Peptide Sequence: LQDVTDDHIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 125
Peptide End: 134

CPTAC ID: non-CPTAC-3896
Peptide Molecular Mass: 1,210.5942
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	15.8	6.9	6.9	12.8	10.3	8.1	20.3	12.4	10.6	15	15	15
y6 (2+)	28.7	15.5	11.1	30.2	14.2	10.4	41.7	21	15.2	15	15	15
y8 (2+)	8.8	6.8	5.6	14.3	8.1	6.7	16.8	10.6	8.7	15	15	15
y3 (1+)	17.1	9.6	7.3	21	9.2	7.4	27.1	13.3	10.4	15	15	15
y6 (1+)	20.8	7.9	6.1	21.2	11.6	8.4	29.7	14	10.4	15	15	15
sum	5.9	4.1	3.9	10.4	6.9	5.4	12	8	6.7	15	15	15

Blvrb

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3896 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Blvrb

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3896 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments