Cd97, CPTAC-3898 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Cd97	N/A

Sequence Length (AA)	Molecular Weight (Da)
818	90413

Protein Name
CD97 antigen

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRGVRCPGLL	VVCILLSLSG	AGTQKAESKN	CAKWCPINSK	CVSNRSCVCK
60	70	80	90	100
PGFSSEKELI	TNPAESCEDI	NECLLPGFSC	GDFAMCKNSE	GSYTCVCNLG
110	120	130	140	150
YKLLSGAESF	VNESENTCQA	SVNTGTTPVP	SRIHTVTTAP	GNLPEQTTTV
160	170	180	190	200
HQTQMGDSEE	RTPKDVNECI	SGQNHCHQST	HCINKLGGYS	CICRQGWKPV
210	220	230	240	250
PGSPNGPVST	VCEDVDECSS	GQHQCHNSTV	CKNTVGSYKC	HCRPGWKPTS
260	270	280	290	300
GSLRGPDTIC	QEPPFPTWTL	LPTAHSQTLL	RFSVEVQNLL	RDFNPATVNY
310	320	330	340	350
TIQKLIEAVD	KLLEDPMETQ	TQQVAAQLLS	NLEQSLRTLA	QFLPKGPFTY
360	370	380	390	400
TSPSNTELSL	MVKEQDNKDV	TTVHHGQTWM	ELDWAVTAGA	KISENGSSVA
410	420	430	440	450
GILSSPNMEK	LLGNTPLNLE	QRRASLEDFY	GSPIPSVSLK	LLSNINSVFL
460	470	480	490	500
TNTNTEKLAS	NVTFKFDFTS	VESIEPRHEL	ICAFWKAHNG	NGYWDTDGCS
510	520	530	540	550
MNGTGFCHCN	HLTSFAILMA	QYHVQDPRLE	LITKVGLLLS	LICLLLCILT
560	570	580	590	600
FLLVKPIQSS	RTMVHLHLCI	CLFLGSIIFL	VGVENEGGEV	GLRCRLVAMM
610	620	630	640	650
LHFCFLAAFC	WMALEGVELY	FLVVRVFQGQ	GLSTWQRCLI	GYGVPLLIVA
660	670	680	690	700
ISMAVVKMDG	YGHATYCWLD	FRKQGFLWSF	SGPVAFIIFC	NAAIFVITVW
710	720	730	740	750
KLTKKFSEIN	PNMKKLRKAR	VLTITAIAQL	LVLGCTWGFG	LFLFNPHSTW
760	770	780	790	800
LSYIFTLLNC	LQGLFLYVML	CLLNKKVREE	YWKWACMVTG	SKYTEFNSST
810	818
TGTGTSQTRA	LRSSESGM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for non-CPTAC-3898 Collapse assay details

Data source: Panorama

Official Gene Symbol: Cd97
Peptide Sequence: TLAQFLPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 338
Peptide End: 345

CPTAC ID: non-CPTAC-3898
Peptide Molecular Mass: 916.5382
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	17.4	4.1	2.2	17.8	6.8	6.4	24.9	7.9	6.8	15	14	15
y3 (1+)	4.7	5.9	7.6	10.8	7.9	9.7	11.8	9.9	12.3	15	14	15
y2 (1+)	4.7	3.1	4.5	5.1	4.4	8.9	6.9	5.4	10	15	14	15
b2 (1+)	8.4	6	2.3	9	12	6.3	12.3	13.4	6.7	15	14	15
b4 (2+)	81.4	95.3	69.9	112.1	125.7	110.5	138.5	157.7	130.8	15	14	15
sum	8.7	4.7	2	8.3	8.2	6.1	12	9.5	6.4	15	14	15

Cd97

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3898 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Cd97

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3898 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments