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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Col11a2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Col11a2 N/A
Sequence Length (AA) Molecular Weight (Da)
1736 171535
Protein Name
Collagen alpha-2(XI) chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MERCSRCHRL LLFLPLVLGL SAAPGWAGAP SVDVLRALRF PSLPDGVRRS
60 70 80 90 100
KGVCPGDVAY RVARPAQLSA PTRQLFPGGF PKDFSLLTVV RTRPGLQAPL
110 120 130 140 150
LTLYSAQGVQ QLGLELGRPV RFLYEDQRGR PQASAQPIFR GLSLADGKWH
160 170 180 190 200
HVAVAVKGQS VTLIVDCKKR VTRPLPRSVH PVLDTHGVVI FGAHILDDEV
210 220 230 240 250
FEGDVQELLV VPGVQAAYQS CGQKDLECER EQRDGPQTQK PHRAQRSPKK
260 270 280 290 300
EPARLHKPQS QEPQKQPTES LYYDYEPPYY DVMTTGTAPD YQYPTPGEEE
310 320 330 340 350
GVLESSPLPF LEEEQTDLQV SPTADSFQAE EYGEGGTDSP AGFYDYTYGY
360 370 380 390 400
GDDYREETEL GPALSAETAH SGAVAHGPRG LKGEKGEPAV LEPGMFVEGP
410 420 430 440 450
PGPEGPAGLA GPPGIQGNPG PVGDPGERGP PGRAGLPGSD GPPGPPGTSL
460 470 480 490 500
MLPFRFGSSG GDKGPVVAAQ EAQAQAILQQ ARLALRGPPG PMGYTGRPGP
510 520 530 540 550
LGQPGSPGLK GESGDLGPQG PRGPQGLTGP PGKAGRRGRA GADGARGMPG
560 570 580 590 600
EPGMKGDRGF DGLPGLPGEK GQRGDTGAQG LPGPPGEDGE RGDDGEIGPR
610 620 630 640 650
GLPGESGPRG LLGPKGPPGI PGPPGVRGMD GPHGPKGSLG PQGEPGPPGQ
660 670 680 690 700
QGTPGAQGLP GPQGAIGPHG EKGARGKPGL PGMPGSDGLP GHPGKEGPPG
710 720 730 740 750
TKGNQGPSGP QGPLGYPGPR GVKGVDGIRG LKGHKGEKGE DGFPGFKGDI
760 770 780 790 800
GVKGDRGEVG VPGSRGEDGP EGPKGRTGPT GDPGPTGLMG EKGKLGVPGL
810 820 830 840 850
PGYPGRQGPK GSLGFPGFPG ASGEKGARGL SGKSGPRGER GPTGPRGQRG
860 870 880 890 900
PRGATGKSGA KGTSGGDGPH GPPGERGLPG PQGPNGFPGP KGPPGPAGKD
910 920 930 940 950
GLPGHPGQRG EVGFQGKTGP PGPPGVVGPQ GTAGESGPMG ERGHSGPPGP
960 970 980 990 1000
PGEQGLPGTS GKEGTKGDPG PPGAPGKDGP AGLRGFPGER GLPGTAGGPG
1010 1020 1030 1040 1050
LKGNEGPAGP PGPAGSPGER GAAGSGGPIG PPGRPGPQGP PGAAGEKGVP
1060 1070 1080 1090 1100
GEKGPIGPTG RDGVQGPVGL PGPAGPPGVA GEDGDKGEVG DPGQKGTKGN
1110 1120 1130 1140 1150
KGEHGPPGPP GPIGPVGQPG AAGADGEPGA RGPQGHFGAK GDEGTRGFNG
1160 1170 1180 1190 1200
PPGPIGLQGL PGPSGEKGET GDGGPMGPPG PPGPRGPAGP NGADGPQGSP
1210 1220 1230 1240 1250
GGVGNLGPPG EKGEPGESGS PGVQGEPGVK GPRGERGEKG ESGQAGEAGP
1260 1270 1280 1290 1300
PGPKGPTGDN GPKGNPGPVG FPGDPGPPGE AGPRGQDGAK GDRGEDGEPG
1310 1320 1330 1340 1350
QPGSPGPTGE NGPPGPLGKR GPAGTPGPEG RQGEKGAKGD PGAVGAPGKT
1360 1370 1380 1390 1400
GPVGPAGLAG KPGPDGLRGL PGSVGQQGRP GATGQAGPPG PVGPPGLPGL
1410 1420 1430 1440 1450
RGDAGAKGEK GHPGLIGLIG PTGEQGEKGD RGLPGPQGSP GQKGETGIPG
1460 1470 1480 1490 1500
ASGPIGPGGP PGLPGPSGPK GAKGATGPAG PKGEKGVQGP PGHPGPPGEV
1510 1520 1530 1540 1550
IQPLPIQMPK KTRRSVDGSK LIQDEEAVPT GGAPGSPAGL EEIFGSLDSL
1560 1570 1580 1590 1600
REEIEQMRRP AGTQDSPART CQDLKLCHPE LPDGEYWVDP NQGCARDAFR
1610 1620 1630 1640 1650
VFCNFTAGGE TCVTPRDDVT QFSYVDSEGS PVGVVQLTFL RLLSVSAHQD
1660 1670 1680 1690 1700
VSYPCSGVSQ DGPLKLRGAN EDELSPETSP YVKEFRDGCQ TQQGRTVLEV
1710 1720 1730 1736
RTPVLEQLPV LDASFADLGA PTRRGGVLLG PVCFMG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3904 Collapse assay details

Data source: Panorama

Official Gene Symbol
Col11a2
Peptide Sequence
GANEDELSPETSPYVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1668
Peptide End
1683
CPTAC ID
non-CPTAC-3904
Peptide Molecular Mass
1,734.7948
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b8 (1+) 13.8 12.7 9.1 16.2 13.9 12.8 21.3 18.8 15.7 15 15 15
b7 (1+) 14.9 13.3 19 19.1 15.7 17.6 24.2 20.6 25.9 15 15 15
y4 (1+) 9.9 8.1 8.3 10.2 7.7 12.1 14.2 11.2 14.7 15 15 15
y8 (1+) 9.4 5 6.7 9.6 8.8 6.4 13.4 10.1 9.3 15 15 15
y9 (1+) 12.8 6.1 5.1 14.1 10.9 8.3 19 12.5 9.7 15 15 15
sum 6.1 3.4 3.5 7.4 7.1 5.6 9.6 7.9 6.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments