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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Dag1 Dag-1
Sequence Length (AA) Molecular Weight (Da)
893 96905
Protein Name
Dystroglycan
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSVDNWLLHP LWGQTFLLLL SVAVAQAHWP SEPSEAVRDW KNQLEASMHS
60 70 80 90 100
VLSDFQEAVP TVVGIPDGTA VVGRSFRVSI PTDLIASSGE IIKVSAAGKE
110 120 130 140 150
ALPSWLHWDP HSHILEGLPL DTDKGVHYIS VSAARLGANG SHVPQTSSVF
160 170 180 190 200
SIEVYPEDHN EPQSVRAASS DPGEVVPSAC AADEPVTVLT VILDADLTKM
210 220 230 240 250
TPKQRIDLLN RMQSFSEVEL HNMKLVPVVN NRLFDMSAFM AGPGNAKKVV
260 270 280 290 300
ENGALLSWKL GCSLNQNSVP DIRGVETPAR EGAMSAQLGY PVVGWHIANK
310 320 330 340 350
KPTLPKRLRR QIHATPTPVT AIGPPTTAIQ EPPSRIVPTP TSPAIAPPTE
360 370 380 390 400
TMAPPVRDPV PGKPTVTIRT RGAIIQTPTL GPIQPTRVSE AGTTVPGQIR
410 420 430 440 450
PTLTIPGYVE PTAVITPPTT TTKKPRVSTP KPATPSTDSS TTTTRRPTKK
460 470 480 490 500
PRTPRPVPRV TTKAPITRLE TASPPTRIRT TTSGVPRGGE PNQRPELKNH
510 520 530 540 550
IDRVDAWVGT YFEVKIPSDT FYDNEDTTTD KLKLTLKLRE QQLVGEKSWV
560 570 580 590 600
QFNSNSQLMY GLPDSSHVGK HEYFMHATDK GGLSAVDAFE IHVHKRPQGD
610 620 630 640 650
KAPARFKARL AGDPAPVVND IHKKIALVKK LAFAFGDRNC SSITLQNITR
660 670 680 690 700
GSIVVEWTNN TLPLEPCPKE QIIGLSRRIA DENGKPRPAF SNALEPDFKA
710 720 730 740 750
LSIAVTGSGS CRHLQFIPVA PPSPGSSAAP ATEVPDRDPE KSSEDDVYLH
760 770 780 790 800
TVIPAVVVAA ILLIAGIIAM ICYRKKRKGK LTLEDQATFI KKGVPIIFAD
810 820 830 840 850
ELDDSKPPPS SSMPLILQEE KAPLPPPEYP NQSMPETTPL NQDTVGEYTP
860 870 880 890 893
LRDEDPNAPP YQPPPPFTAP MEGKGSRPKN MTPYRSPPPY VPP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3909 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dag1
Peptide Sequence
LVPVVNNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
225
Peptide End
232
CPTAC ID
non-CPTAC-3909
Peptide Molecular Mass
909.5396
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 19.3 6.1 4.6 24.8 7 4.6 31.4 9.3 6.5 15 15 15
y6 (2+) 6.4 5 2.9 7.2 9.5 4 9.6 10.7 4.9 15 15 15
y4 (1+) 23.7 6.8 3 34.5 10.2 4 41.9 12.3 5 15 15 15
y5 (1+) 48.3 4.8 5.3 59.6 6.6 5.8 76.7 8.2 7.9 15 15 15
y6 (1+) 19.1 5 4.4 29.4 9.8 4.9 35.1 11 6.6 15 15 15
sum 8.3 3.8 2.3 11 8.4 3 13.8 9.2 3.8 15 15 15

Additional Resources and Comments