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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Dpysl2 Crmp2, Ulip2
Sequence Length (AA) Molecular Weight (Da)
572 62278
Protein Name
Dihydropyrimidinase-related protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSYQGKKNIP RITSDRLLIK GGKIVNDDQS FYADIYMEDG LIKQIGENLI
60 70 80 90 100
VPGGVKTIEA HSRMVIPGGI DVHTRFQMPD QGMTSADDFF QGTKAALAGG
110 120 130 140 150
TTMIIDHVVP EPGTSLLAAF DQWREWADSK SCCDYSLHVD ITEWHKGIQE
160 170 180 190 200
EMEALVKDHG VNSFLVYMAF KDRFQLTDSQ IYEVLSVIRD IGAIAQVHAE
210 220 230 240 250
NGDIIAEEQQ RILDLGITGP EGHVLSRPEE VEAEAVNRSI TIANQTNCPL
260 270 280 290 300
YVTKVMSKSA AEVIAQARKK GTVVYGEPIT ASLGTDGSHY WSKNWAKAAA
310 320 330 340 350
FVTSPPLSPD PTTPDFLNSL LSCGDLQVTG SAHCTFNTAQ KAVGKDNFTL
360 370 380 390 400
IPEGTNGTEE RMSVIWDKAV VTGKMDENQF VAVTSTNAAK VFNLYPRKGR
410 420 430 440 450
ISVGSDADLV IWDPDSVKTI SAKTHNSALE YNIFEGMECR GSPLVVISQG
460 470 480 490 500
KIVLEDGTLH VTEGSGRYIP RKPFPDFVYK RIKARSRLAE LRGVPRGLYD
510 520 530 540 550
GPVCEVSVTP KTVTPASSAK TSPAKQQAPP VRNLHQSGFS LSGAQIDDNI
560 570 572
PRRTTQRIVA PPGGRANITS LG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-61 Collapse assay details

Data source: Panorama

Official Gene Symbol
DPYSL2
Peptide Modified Sequence
GLYDGPVC[+57.0]EVSVTPK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
504
Peptide - Site of Modification
8
Peptide Start
497
Peptide End
511
CPTAC ID
CPTAC-61
Peptide Molecular Mass
1,619.7865
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 26.6 14.6 12.9 30.8 15.6 21.4 40.7 21.4 25 15 25 15
y10 (1+) 11.6 17.1 12.3 12.3 16.9 16.8 16.9 24 20.8 15 25 15
y8 (1+) 12.7 15 23.1 22.2 15.1 24.4 25.6 21.3 33.6 15 25 15
sum 11.7 9.2 12.6 14 11.8 14.5 18.2 15 19.2 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-60 Collapse assay details

Data source: Panorama

Official Gene Symbol
DPYSL2
Peptide Sequence
IAVGSDADLVIWDPDSVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
401
Peptide End
418
CPTAC ID
CPTAC-60
Peptide Molecular Mass
1,898.9626
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 28.4 30.6 30.7 40.9 45.6 38.7 49.8 54.9 49.4 15 15 15
y9 (1+) 49.2 19.1 32.7 109.5 29.4 35.1 120 35.1 48 15 15 15
y8 (1+) 49.1 29.8 20.3 107.7 36.5 23.2 118.4 47.1 30.8 15 15 15
sum 36.4 22.2 23 90.4 28.7 25.3 97.5 36.3 34.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3911 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dpysl2
Peptide Sequence
NLHQSGFSLSGAQIDDNIPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
533
Peptide End
552
CPTAC ID
non-CPTAC-3911
Peptide Molecular Mass
2,168.0610
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b18 (2+) 9.5 8.5 8 10.9 9.5 8.3 14.5 12.7 11.5 15 15 15
b14 (2+) 38.6 12.4 25 42.1 12.3 30.6 57.1 17.5 39.5 15 15 15
b13 (2+) 27.6 7.4 7.6 34.2 11.8 9.7 43.9 13.9 12.3 15 15 15
y2 (1+) 8.7 6.5 5.6 10.5 10.3 6.1 13.6 12.2 8.3 15 15 15
y6 (1+) 14.6 5.8 4.4 15.9 9.1 7.3 21.6 10.8 8.5 15 15 15
sum 15 3 3.4 17.5 5.9 5.4 23 6.6 6.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3426 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dpysl2
Peptide Sequence
VFNLYPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
391
Peptide End
397
CPTAC ID
non-CPTAC-3426
Peptide Molecular Mass
907.4916
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.4 5.9 8.2 15 10.3 6.3 15.6 11.9 10.3 15 15 15
y2 (1+) 10 9 9.5 13 10.7 9.6 16.4 14 13.5 15 15 15
y3 (1+) 14.6 6.9 6.9 20.8 10 7.5 25.4 12.1 10.2 15 15 15
y4 (1+) 5.4 8.3 4.3 18.2 9.2 8 19 12.4 9.1 15 15 15
y5 (1+) 7.5 6.8 6.5 17.1 11 5.5 18.7 12.9 8.5 15 15 15
sum 3 3.9 3.5 15.2 8.9 3.6 15.5 9.7 5 15 15 15


Additional Resources and Comments