Ext1, CPTAC-3915 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Ext1	N/A

Sequence Length (AA)	Molecular Weight (Da)
746	86308

Protein Name
Exostosin-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQAKKRYFIL	LSAGSCLALL	FYFGGVQFRA	SRSHSRREEH	SGRNGLHQPS
60	70	80	90	100
PDHFWPRFPD	ALRPFFPWDQ	LENEDSSVHI	SPRQKRDANS	SIYKGKKCRM
110	120	130	140	150
ESCFDFTLCK	KNGFKVYVYP	QQKGEKIAES	YQNILAAIEG	SRFYTSDPSQ
160	170	180	190	200
ACLFVLSLDT	LDRDQLSPQY	VHNLRSKVQS	LHLWNNGRNH	LIFNLYSGTW
210	220	230	240	250
PDYTEDVGFD	IGQAMLAKAS	ISTENFRPNF	DVSIPLFSKD	HPRTGGERGF
260	270	280	290	300
LKFNTIPPLR	KYMLVFKGKR	YLTGIGSDTR	NALYHVHNGE	DVLLLTTCKH
310	320	330	340	350
GKDWQKHKDS	RCDRDNTEYE	KYDYREMLHN	ATFCLVPRGR	RLGSFRFLEA
360	370	380	390	400
LQAACVPVML	SNGWELPFSE	VINWNQAAVI	GDERLLLQIP	STIRSIHQDK
410	420	430	440	450
ILALRQQTQF	LWEAYFSSVE	KIVLTTLEII	QDRIFKHISR	NSLIWNKHPG
460	470	480	490	500
GLFVLPQYSS	YLGDFPYYYA	NLGLKPPSKF	TAVIHAVTPL	VSQSQPVLKL
510	520	530	540	550
LVAAAKSQYC	AQIIVLWNCD	KPLPAKHRWP	ATAVPVIVIE	GESKVMSSRF
560	570	580	590	600
LPYDNIITDA	VLSLDEDTVL	STTEVDFAFT	VWQSFPERIV	GYPARSHFWD
610	620	630	640	650
NSKERWGYTS	KWTNDYSMVL	TGAAIYHKYY	HYLYSHYLPA	SLKNMVDQLA
660	670	680	690	700
NCEDILMNFL	VSAVTKLPPI	KVTQKKQYKE	TMMGQTSRAS	RWADPDHFAQ
710	720	730	740	746
RQSCMNTFAS	WFGYMPLIHS	QMRLDPVLFK	DQVSILRKKY	RDIERL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3915 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ext1
Peptide Sequence: FTAVIHAVTPLVSQSQPVLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 480
Peptide End: 499

CPTAC ID: non-CPTAC-3915
Peptide Molecular Mass: 2,134.2150
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	8.9	3.1	3.3	11.9	9.2	8.4	14.9	9.7	9	15	15	15
y8 (1+)	6.4	3.1	2.1	10.1	9.8	9.6	12	10.3	9.8	15	15	15
b19 (2+)	5.4	3.9	2.8	6.9	10.9	9.5	8.8	11.6	9.9	15	15	15
y11 (2+)	4.7	3	2.9	7.8	8.2	10.2	9.1	8.7	10.6	15	15	15
y4 (1+)	5.7	4.2	3.6	8.8	10.4	10.7	10.5	11.2	11.3	15	15	15
sum	3.7	1.6	1.7	6.7	9.1	9.4	7.7	9.2	9.6	15	15	15

Ext1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3915 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Ext1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3915 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments