Ighm, CPTAC-3919 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Ighm	Igh-6

Sequence Length (AA)	Molecular Weight (Da)
454	49972

Protein Name
Immunoglobulin heavy constant mu

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
SQSFPNVFPL	VSCESPLSDK	NLVAMGCLAR	DFLPSTISFT	WNYQNNTEVI
60	70	80	90	100
QGIRTFPTLR	TGGKYLATSQ	VLLSPKSILE	GSDEYLVCKI	HYGGKNRDLH
110	120	130	140	150
VPIPAVAEMN	PNVNVFVPPR	DGFSGPAPRK	SKLICEATNF	TPKPITVSWL
160	170	180	190	200
KDGKLVESGF	TTDPVTIENK	GSTPQTYKVI	STLTISEIDW	LNLNVYTCRV
210	220	230	240	250
DHRGLTFLKN	VSSTCAASPS	TDILTFTIPP	SFADIFLSKS	ANLTCLVSNL
260	270	280	290	300
ATYETLNISW	ASQSGEPLET	KIKIMESHPN	GTFSAKGVAS	VCVEDWNNRK
310	320	330	340	350
EFVCTVTHRD	LPSPQKKFIS	KPNEVHKHPP	AVYLLPPARE	QLNLRESATV
360	370	380	390	400
TCLVKGFSPA	DISVQWLQRG	QLLPQEKYVT	SAPMPEPGAP	GFYFTHSILT
410	420	430	440	450
VTEEEWNSGE	TYTCVVGHEA	LPHLVTERTV	DKSTGKPTLY	NVSLIMSDTG
454
GTCY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2676 Collapse assay details

Data source: Panorama

Official Gene Symbol: IGHM
Peptide Sequence: GFPSVLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 55
Peptide End: 61

CPTAC ID: non-CPTAC-2676
Peptide Molecular Mass: 774.4388
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	4.7	4.4	4.5	13.8	8.5	6.8	14.6	9.6	8.2	15	15	15
y4 (1+)	5.6	5.3	4.9	11.9	9.7	7.1	13.2	11.1	8.6	15	15	15
y5 (1+)	4.7	5.6	4.7	9.7	6.9	5.8	10.8	8.9	7.5	15	15	15
y5 (2+)	4.3	6.9	4.5	13.9	9.1	7.2	14.5	11.4	8.5	15	15	15
y2 (1+)	7.4	3.3	6.4	10.8	5.3	6.4	13.1	6.2	9.1	15	15	15
sum	2.4	4.8	2.6	12.5	8	5.6	12.7	9.3	6.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3919 Collapse assay details

Data source: Panorama

Official Gene Symbol: Ighm
Peptide Sequence: LVESGFTTDPVTIENK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 155
Peptide End: 170

CPTAC ID: non-CPTAC-3919
Peptide Molecular Mass: 1,748.8832
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.