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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Mmp17

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Mmp17 Mt4mmp
Sequence Length (AA) Molecular Weight (Da)
578 64333
Protein Name
Matrix metalloproteinase-17
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGRRPRGPGS PRGPGPPRPG PGLPPLLLVL ALAAHGGCAA PAPRAEDLSL
60 70 80 90 100
GVEWLSRFGY LPPADPASGQ LQTQEELSKA ITAMQQFGGL ETTGILDEAT
110 120 130 140 150
LALMKTPRCS LPDLPPGAQS RRKRQTPPPT KWSKRNLSWR VRTFPRDSPL
160 170 180 190 200
GRDTVRALMY YALKVWSDIT PLNFHEVAGN AADIQIDFSK ADHNDGYPFD
210 220 230 240 250
GPGGTVAHAF FPGDHHTAGD THFDDDEPWT FRSSDAHGMD LFAVAVHEFG
260 270 280 290 300
HAIGLSHVAA PSSIMQPYYQ GPVGDPLRYG LPYEDRVRVW QLYGVRESVS
310 320 330 340 350
PTAQLDTPEP EEPPLLPEPP NNRSSTPPQK DVPHRCTAHF DAVAQIRGEA
360 370 380 390 400
FFFKGKYFWR LTRDRHLVSL QPAQMHRFWR GLPLHLDSVD AVYERTSDHK
410 420 430 440 450
IVFFKGDRYW VFKDNNVEEG YPRPVSDFSL PPGGIDAVFS WAHNDRTYFF
460 470 480 490 500
KDQLYWRYDD HTRRMDPGYP AQGPLWRGVP SMLDDAMRWS DGASYFFRGQ
510 520 530 540 550
EYWKVLDGEL EAAPGYPQST ARDWLVCGEP LADAEDVGPG PQGRSGAQDG
560 570 578
LAVCSCTSDA HRLALPSLLL LTPLLWGL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3927 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mmp17
Peptide Sequence
YGLPYEDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
279
Peptide End
286
CPTAC ID
non-CPTAC-3927
Peptide Molecular Mass
1,011.4662
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 15.4 5 3.5 18.2 8.5 6.7 23.8 9.9 7.6 15 15 15
b2 (1+) 16.2 6.1 4.6 43.9 9.3 5.1 46.8 11.1 6.9 15 15 15
y5 (2+) 12.7 7.4 5.7 13.8 8.3 7.8 18.8 11.1 9.7 15 15 15
y6 (2+) 19.2 7.2 7 37.1 11.3 13 41.8 13.4 14.8 15 15 15
y5 (1+) 9.9 4 4.7 14.8 5.4 6 17.8 6.7 7.6 15 15 15
sum 8.4 2.4 2.8 21.2 5.1 5.1 22.8 5.6 5.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments