Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Mrc1

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Mrc1 N/A
Sequence Length (AA) Molecular Weight (Da)
1456 164981
Protein Name
Macrophage mannose receptor 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLLLLLAFI SVIPVSVQLL DARQFLIYNE DHKRCVDALS AISVQTATCN
60 70 80 90 100
PEAESQKFRW VSDSQIMSVA FKLCLGVPSK TDWASVTLYA CDSKSEYQKW
110 120 130 140 150
ECKNDTLFGI KGTELYFNYG NRQEKNIKLY KGSGLWSRWK VYGTTDDLCS
160 170 180 190 200
RGYEAMYSLL GNANGAVCAF PFKFENKWYA DCTSAGRSDG WLWCGTTTDY
210 220 230 240 250
DKDKLFGFCP LHFEGSERLW NKDPLTGILY QINSKSALTW HQARASCKQQ
260 270 280 290 300
NADLLSVTEI HEQMYLTGLT SSLSSGLWIG LNSLSVRSGW QWAGGSPFRY
310 320 330 340 350
LNWLPGSPSS EPGKSCVSLN PGKNAKWENL ECVQKLGYIC KKGNNTLNPF
360 370 380 390 400
IIPSASDVPT GCPNQWWPYA GHCYRIHREE KKIQKYALQA CRKEGGDLAS
410 420 430 440 450
IHSIEEFDFI FSQLGYEPND ELWIGLNDIK IQMYFEWSDG TPVTFTKWLP
460 470 480 490 500
GEPSHENNRQ EDCVVMKGKD GYWADRACEQ PLGYICKMVS QSHAVVPEGA
510 520 530 540 550
DKGCRKGWKR HGFYCYLIGS TLSTFTDANH TCTNEKAYLT TVEDRYEQAF
560 570 580 590 600
LTSLVGLRPE KYFWTGLSDV QNKGTFRWTV DEQVQFTHWN ADMPGRKAGC
610 620 630 640 650
VAMKTGVAGG LWDVLSCEEK AKFVCKHWAE GVTRPPEPTT TPEPKCPENW
660 670 680 690 700
GTTSKTSMCF KLYAKGKHEK KTWFESRDFC KAIGGELASI KSKDEQQVIW
710 720 730 740 750
RLITSSGSYH ELFWLGLTYG SPSEGFTWSD GSPVSYENWA YGEPNNYQNV
760 770 780 790 800
EYCGELKGDP GMSWNDINCE HLNNWICQIQ KGKTLLPEPT PAPQDNPPVT
810 820 830 840 850
ADGWVIYKDY QYYFSKEKET MDNARAFCKK NFGDLATIKS ESEKKFLWKY
860 870 880 890 900
INKNGGQSPY FIGMLISMDK KFIWMDGSKV DFVAWATGEP NFANDDENCV
910 920 930 940 950
TMYTNSGFWN DINCGYPNNF ICQRHNSSIN ATAMPTTPTT PGGCKEGWHL
960 970 980 990 1000
YKNKCFKIFG FANEEKKSWQ DARQACKGLK GNLVSIENAQ EQAFVTYHMR
1010 1020 1030 1040 1050
DSTFNAWTGL NDINAEHMFL WTAGQGVHYT NWGKGYPGGR RSSLSYEDAD
1060 1070 1080 1090 1100
CVVVIGGNSR EAGTWMDDTC DSKQGYICQT QTDPSLPVSP TTTPKDGFVT
1110 1120 1130 1140 1150
YGKSSYSLMK LKLPWHEAET YCKDHTSLLA SILDPYSNAF AWMKMHPFNV
1160 1170 1180 1190 1200
PIWIALNSNL TNNEYTWTDR WRVRYTNWGA DEPKLKSACV YMDVDGYWRT
1210 1220 1230 1240 1250
SYCNESFYFL CKKSDEIPAT EPPQLPGKCP ESEQTAWIPF YGHCYYFESS
1260 1270 1280 1290 1300
FTRSWGQASL ECLRMGASLV SIETAAESSF LSYRVEPLKS KTNFWIGMFR
1310 1320 1330 1340 1350
NVEGKWLWLN DNPVSFVNWK TGDPSGERND CVVLASSSGL WNNIHCSSYK
1360 1370 1380 1390 1400
GFICKMPKII DPVTTHSSIT TKADQRKMDP QPKGSSKAAG VVTVVLLIVI
1410 1420 1430 1440 1450
GAGVAAYFFY KKRHALHIPQ EATFENTLYF NSNLSPGTSD TKDLMGNIEQ
1456
NEHAII

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3930 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mrc1
Peptide Sequence
AYLTTVEDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
537
Peptide End
545
CPTAC ID
non-CPTAC-3930
Peptide Molecular Mass
1,066.5295
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 18 4.9 5.3 19.3 7.5 7.8 26.4 9 9.4 15 15 15
b2 (1+) 7.1 4.8 3.6 12.7 8.1 4.6 14.5 9.4 5.8 15 15 15
y5 (1+) 12.9 6.8 5.9 13.7 10.6 6.7 18.8 12.6 8.9 15 15 15
y6 (1+) 9.3 4.6 3.4 12.8 6.1 4.6 15.8 7.6 5.7 15 15 15
y7 (1+) 13.3 8.4 8.9 15.2 7.4 8.2 20.2 11.2 12.1 15 15 15
sum 5.5 2.2 1.7 7.9 6.3 3.2 9.6 6.7 3.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments