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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ptk7 N/A
Sequence Length (AA) Molecular Weight (Da)
1062 117532
Protein Name
Inactive tyrosine-protein kinase 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGARPLTLLR ALLLPLLAGA QAAIVFIKEP SSQDALQGRR ALLRCEVEAP
60 70 80 90 100
DPVHVYWLLN GVPVQDTERR FAQGSSLSFA AVDRLQDSGA FQCVARDNVT
110 120 130 140 150
GEEVRSTNAS FNIKWIEAGP VVLKHPASEA EIQPQTQVTL RCHIDGHPRP
160 170 180 190 200
TYQWFRDGTP LSDDQSTHTV SSRERNLTLR PASPEHSGLY SCCAHNAFGQ
210 220 230 240 250
ACSSQNFTLS VADESFARVV LAPQDVVVAR NEEAMFHCQF SAQPPPSLQW
260 270 280 290 300
VFEDETPITN RSRPPHLRRA VVFANGSLLL TQVRPRNAGV YRCIGQGQRG
310 320 330 340 350
PPIVLEATLH LAEIEDMLPF EPRVFIAGDE ERVTCPAPQG LPTPSVWWEH
360 370 380 390 400
AGVPLPAHGR VHQKGLELVF VTIAESDTGV YTCHASNLAG QRRQDVNITV
410 420 430 440 450
ATVPTWLRKP QDSQLEEGKP GYLHCLTQAT PKPTVIWYRN QMLISEDSRF
460 470 480 490 500
EVSKNGTLRI NSVEVYDGTL YRCVSSTPAG SIEAQARVQV LEKLKFTPPP
510 520 530 540 550
QPQQCMEFDK EATVPCSATG REKPTVKWVR ADGSSLPEWV TDNAGTLHFA
560 570 580 590 600
RVTRDDAGNY TCIASNEPQG QIRAHVQLTV AVFITFKVEP ERTTVYQGHT
610 620 630 640 650
ALLRCEAQGD PKPLIQWKGK DRILDPTKLG PRMHIFQNGS LVIHDVAPED
660 670 680 690 700
SGSYTCIAGN SCNIRHTEAP LLVVDKPVME DSEGPGSPPP YKMIQTIGLS
710 720 730 740 750
VGAAVAYIIA VLGLMFYCKK RCKAKRLQKQ PEGEEPEMEC LNGGPLQNGQ
760 770 780 790 800
PSAEIQEEVA LTSLGSGPPA TNKRHSAGDR MHFPRASLQP ITTLGKSEFG
810 820 830 840 850
EVFLAKAQGV EEGATETLVL VKSLQSRDEQ QQLDFRREVE MFGKLNHANV
860 870 880 890 900
VRLLGLCREA EPHYMVLEYV DLGDLKQFLR ISKNKDEKLK SQPLSTKQKV
910 920 930 940 950
ALCSQVALGM EHLSNNRFVH KDLAARNCLI SAQRQVKVSA LGLSKDVYNS
960 970 980 990 1000
EYYHFRQAWV PLRWMSPEAV LEGDFSTKSD VWAFGVLMWE VFTHGEMPHG
1010 1020 1030 1040 1050
GQADDEVLAD LQAGKARLPQ PEGCPSKLYR LMQRCWAPNP KDRPSFSEIA
1060 1062
STLGDSPADS KQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3932 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ptk7
Peptide Sequence
VVLAPQDVVVAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
219
Peptide End
230
CPTAC ID
non-CPTAC-3932
Peptide Molecular Mass
1,264.7503
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 5 2.5 3.4 8 3.2 5.6 9.4 4.1 6.6 15 15 15
y10 (2+) 7.1 3.3 3 6.6 7.1 6.6 9.7 7.8 7.2 15 15 15
y8 (2+) 8 4.8 2 14.3 5 6.1 16.4 6.9 6.4 15 15 15
b3 (1+) 7 4.9 2.3 15.7 6.5 6.6 17.2 8.1 7 15 15 15
b4 (1+) 10.2 4.1 2.9 22.9 6.8 7.5 25.1 7.9 8 15 15 15
sum 5.7 2.4 1.2 11.6 4.5 5.6 12.9 5.1 5.7 15 15 15

Additional Resources and Comments