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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Prg4 Msf, Szp
Sequence Length (AA) Molecular Weight (Da)
1054 115996
Protein Name
Proteoglycan 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGWKILPVCL SLLLPVVLIQ QVSSQDLSSC AGRCGEGYSR DATCNCDYNC
60 70 80 90 100
QHYMECCPDF KRVCSPELSC KGRCFESFAR GRECDCDSQC KQYGKCCADY
110 120 130 140 150
DSFCEEVHNS TSPSSKTAPT PAGASDTIKS TTKRSPKSPT TRTIKVVESE
160 170 180 190 200
ELTEEHSDSE NQESSSSSSS SSSTIRKIKS SKNSANRELQ KNPNVKDNKK
210 220 230 240 250
NTPKKKPNPE PPAVDEAGSG LDNGEFKLTP PPPDPPTTPH SKVATSPKTT
260 270 280 290 300
AAKPVTPKPS LAPNSETSKE ASLASNKETT VETKETTATN KQSSASKKKT
310 320 330 340 350
TSVKETRSAE KTSDKDVEPT STTPKNSAPT TTKKPVTTTK ESKFLPLPQE
360 370 380 390 400
PEPTTAKEPP PTTKKPEPTT RKEPEPTTPK EPEPTTPKEP EPTTPKEPEP
410 420 430 440 450
TTPKEPPPTT KKPEPTTPKE PGPTTPKEPE PTTTKEPEPT TTKEPESTTR
460 470 480 490 500
KEPEPTTPKE PEPTTPKEPE PTTLKEPEPT TPKEPEPTTP KEPEPTTPKE
510 520 530 540 550
PEPTTPKEPE PTTPKEPEPT TPKEPEPTTP KEPEPTTPKE PEPTTPKKPE
560 570 580 590 600
PTTPKEPVPT TPKEPEPTTP KEPEPTTPKE PEPTTRKEPE PTTPKEPEPT
610 620 630 640 650
TPKEPEPTTP KKPEPTTTSP KTTTLKATTL APKVTAPAEE IQNKPEETTP
660 670 680 690 700
ASEDSDDSKT TLKPQKPTKA PKPTKKPTKA PKKPTSTKKP KTPKTRKPKT
710 720 730 740 750
TPSPLKTTSA TPELNTTPLE VMLPTTTIPK QTPNPETAEV NPDHEDADGG
760 770 780 790 800
EGEKPLIPGP PVLFPTAIPG TDLLAGRLNQ GININPMLSD ETNLCNGKPV
810 820 830 840 850
DGLTTLRNGT LVAFRGHYFW MLNPFRPPSP PRRITEVWGI PSPIDTVFTR
860 870 880 890 900
CNCEGKTFFF KDSQYWRFTN DVVDPGYPKQ IVKGFGGLTG KIVAALSIAK
910 920 930 940 950
YKDRPESVYF FKRGGNIQQY TYKQEPMKKC TGRRPAINYS VYGEAAQVRR
960 970 980 990 1000
RRFERAVGPF QTHTFRIHYS VPMRVSYQDK GFLHNEVKVS TMWRGFPNVV
1010 1020 1030 1040 1050
TSAITLPNIR KPDGYDYYAF SKDQYYNIDV PTRTARAITT RSGQTLSKIW
1054
YNCP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3938 Collapse assay details

Data source: Panorama

Official Gene Symbol
Prg4
Peptide Sequence
GFPNVVTSAITLPNIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
995
Peptide End
1010
CPTAC ID
non-CPTAC-3938
Peptide Molecular Mass
1,697.9465
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 11 4.5 2.6 11.7 14.3 14.7 16.1 15 14.9 15 15 15
y10 (1+) 6.7 4.3 2.3 13.7 12.9 13.8 15.3 13.6 14 15 15 15
y6 (1+) 23 3.1 2.5 43.5 12.4 13.1 49.2 12.8 13.3 15 15 15
y4 (1+) 7.7 2.5 2.5 9.6 12.5 14.1 12.3 12.7 14.3 15 15 15
y14 (2+) 18.3 3.4 3.4 82.5 15.2 13.9 84.5 15.6 14.3 15 15 15
sum 10.4 1.3 1.3 44.4 13 13.8 45.6 13.1 13.9 15 15 15


Additional Resources and Comments