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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ywhag N/A
Sequence Length (AA) Molecular Weight (Da)
247 28303
Protein Name
14-3-3 protein gamma
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK
60 70 80 90 100
NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV
110 120 130 140 150
LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS
160 170 180 190 200
EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA
210 220 230 240 247
FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-450 Collapse assay details

Data source: Panorama

Official Gene Symbol
YWHAG
Peptide Sequence
NVTELNEPLSNEER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
29
Peptide End
42
CPTAC ID
CPTAC-450
Peptide Molecular Mass
1,642.7798
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 10.4 5.9 3.9 17.8 6.7 7 20.6 8.9 8 15 25 15
y7 (1+) 7.9 5.5 2.9 8.1 6.8 5.4 11.3 8.7 6.1 15 25 15
y12 (2+) 17.2 10.9 7.6 20.9 11.2 5.7 27.1 15.6 9.5 15 25 15
sum 6.9 5 3.2 8.2 6.5 5.7 10.7 8.2 6.5 15 25 15

Additional Resources and Comments


Assay Details for CPTAC-451 Collapse assay details

Data source: Panorama

Official Gene Symbol
YWHAG
Peptide Sequence
TAFDDAIAELDTLNEDSYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
199
Peptide End
217
CPTAC ID
CPTAC-451
Peptide Molecular Mass
2,129.9641
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Broad Institute and FHCRC
Submitting Lab PI
Steve Carr

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 22.9 21.2 21.2 24.5 35 33.4 33.5 40.9 39.6 25 15 15
y9 (1+) 20.8 19 13.8 31.8 31.9 35.5 38 37.1 38.1 25 15 15
y8 (1+) 24.7 13.3 13.9 28.8 29.7 35.9 37.9 32.5 38.5 25 15 15
sum 17.9 12.9 11.6 25 29.7 33.8 30.7 32.4 35.7 25 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3956 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ywhag
Peptide Sequence
AYSEAHEISK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
153
Peptide End
162
CPTAC ID
non-CPTAC-3956
Peptide Molecular Mass
1,133.5353
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 10.7 3.4 3.2 15.5 6.8 4.3 18.8 7.6 5.4 15 15 15
y8 (2+) 7 3.3 2.2 9.8 4.5 3.7 12 5.6 4.3 15 15 15
y6 (2+) 26.1 6.5 6.6 26.9 5.7 5.8 37.5 8.6 8.8 15 15 15
y5 (3+) 137.5 142.5 95.2 172.7 189 120.5 220.8 236.7 153.6 15 15 15
b2 (1+) 13.3 4.1 3.9 11.6 5.1 5.5 17.6 6.5 6.7 15 15 15
sum 5 2.1 1.5 8.1 4.2 3.4 9.5 4.7 3.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3957 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ywhag
Peptide Sequence
YLAEVATGEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
133
Peptide End
142
CPTAC ID
non-CPTAC-3957
Peptide Molecular Mass
1,079.5499
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 7.4 2.7 1.3 16.2 4.9 3.6 17.8 5.6 3.8 15 15 15
y3 (1+) 15.4 4.4 2.8 20.2 6.3 5.1 25.4 7.7 5.8 15 15 15
y4 (1+) 18.4 5.7 2.1 19.3 7.4 3.7 26.7 9.3 4.3 15 15 15
y7 (1+) 11.1 4.9 4.1 15.8 8 5.2 19.3 9.4 6.6 15 15 15
y8 (1+) 10.3 2.4 2.1 16.1 5.8 4.8 19.1 6.3 5.2 15 15 15
sum 9.8 2 1 15 4.9 3.1 17.9 5.3 3.3 15 15 15

Additional Resources and Comments