Khdc3, CPTAC-3979 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Khdc3	N/A

Sequence Length (AA)	Molecular Weight (Da)
440	47995

Protein Name
KH domain-containing protein 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MASLKRFQTL	VPLDHKQGTL	FEIIGEPKLP	KWFHVECLED	PKRLYVEPRL
60	70	80	90	100
LEIMFGKDGE	HIPHLESMLH	TLIHVNVWGP	ERRAEIWIFG	PPPFRRDVDR
110	120	130	140	150
MLTDLAHYCR	MKLMEIEALE	AGVERRRMAA	HKAATQPAPV	KVREAAPRPA
160	170	180	190	200
SVKVPETATQ	PAPVKVREAA	PQPAPVQEVR	EAAPQQASVQ	EEVREAATEQ
210	220	230	240	250
APVQEVREAA	TEQAPVQEVS	EAATEQAPVQ	EVNEAATEQA	SVQAVREAAT
260	270	280	290	300
RPAPGKVRKA	ATQPAPVQVC	QEATQLAPVK	VREAATQPAS	GKVREAATQL
310	320	330	340	350
APVKVRKAAT	QLAPVKVHEA	ATQPAPGKVS	DAATQSASVQ	VREAATQLSP
360	370	380	390	400
VEATDTSQLA	QVKADEAFAQ	HTSGEAHQVA	NGQSPIEVCE	TATGQHSLDV
410	420	430	440
SRALSQKCPE	VFEWETQSCL	DGSYVIVQPP	RDAWESFIIL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3979 Collapse assay details

Data source: Panorama

Official Gene Symbol: Khdc3
Peptide Sequence: EAATEQAPVQEVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 195
Peptide End: 207

CPTAC ID: non-CPTAC-3979
Peptide Molecular Mass: 1,426.7052
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	27.7	6.6	7.3	26.3	9.9	9.5	38.2	11.9	12	15	15	15
y2 (1+)	25.3	9.5	9	26.5	13.4	9.2	36.6	16.4	12.9	15	15	15
y6 (1+)	10	6.4	4.4	8.1	8.7	5.7	12.9	10.8	7.2	15	15	15
y7 (1+)	16.1	7.5	4.5	17.8	7.2	7.5	24	10.4	8.7	15	15	15
y8 (1+)	18.4	10.4	7.6	26.8	6.7	10.1	32.5	12.4	12.6	15	15	15
sum	8.7	3.7	3.2	8.5	6.5	4.9	12.2	7.5	5.9	15	15	15

Khdc3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3979 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Khdc3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3979 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments