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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Fbln2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Fbln2 N/A
Sequence Length (AA) Molecular Weight (Da)
1221 131834
Protein Name
Fibulin-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLLQESAGVW LALALVTALT PSPSMAVPWQ DCTGAECPLL ENCIEEALEP
60 70 80 90 100
GACCATCVQQ GCACEGYQYY DCVQGGFVDG RVPAGQSYFV DFGSTECSCP
110 120 130 140 150
PGGGKISCQF MLCPELPPNC IEAVVVADSC PQCGQVGCVH SGRKYAAGHT
160 170 180 190 200
VHLSSCRACH CPDAGGELIC YQLPGCHGNF SDAEEGDSER QYEDPYSYDQ
210 220 230 240 250
EVAEAEATTA IVNEVQAGAE GPPAALGGGN LPPSSIRVTP WPVALPRPTA
260 270 280 290 300
AAALGPPAPV QAKARRVTLD TEEDEEEEEE ETLVTEPPTA GSPGRLDSLP
310 320 330 340 350
TRSPARPGFP VQEKEAEAKA GPEENLIPDA QVTPRSVMQE GAAPLPRSGL
360 370 380 390 400
AALSPSLATD SSSEDPVKPS DHPTLSTLPP DRAQVSPSPE TPEEIPQHPQ
410 420 430 440 450
LLPRFRAEED IDPNSVHSVP RGDLDGSTKD LIETCCAAGQ QWAIDNDECQ
460 470 480 490 500
EIPENGAQSD ICRIAQRQCC ISYLKEKSCV AGVMGAKEGE TCGAEDNDTC
510 520 530 540 550
GVSLYKQCCD CCGLGLRVRA EGQSCESNPN LGYPCNHVML SCCEGEEPLI
560 570 580 590 600
VPEVRRPPEP EAAPRRVSEM EMASREALSL GTEAELPNSL PGDDQDECLM
610 620 630 640 650
LPGELCQHLC INTVGSYRCA CFPGFELQGD GRTCRPDRGA PQLDTARESA
660 670 680 690 700
PRSESAQVSP NTIPLPVPQP NTCKDNGPCR QVCRVVGDTA MCSCFPGYAI
710 720 730 740 750
MADGVSCEDQ DECLMGTHDC SWKQFCVNTL GSFYCVNHTV LCAEGYILNA
760 770 780 790 800
HRKCVDINEC VTDLHTCTRA EHCVNTPGSF QCYKALTCEP GYVLTDGECT
810 820 830 840 850
DVDECVTGTH NCQAGFSCQN TKGSFYCQAR QRCMDGFLQD PEGNCVDINE
860 870 880 890 900
CTSLLEPCRS GFSCINTVGS YTCQRNPLVC GRGYHANEEG SECVDVNECE
910 920 930 940 950
TGVHRCGEGQ LCYNLPGSYR CDCKPGFQRD AFGRTCIDVN ECWVSPGRLC
960 970 980 990 1000
QHTCENTPGS YRCSCAAGFL LAADGKHCED VNECETRRCS QECANIYGSY
1010 1020 1030 1040 1050
QCYCRQGYQL AEDGHTCTDI DECAQGAGIL CTFRCVNVPG SYQCACPEQG
1060 1070 1080 1090 1100
YTMMANGRSC KDLDECALGT HNCSEAETCH NIQGSFRCLR FDCPPNYVRV
1110 1120 1130 1140 1150
SETKCERTTC QDITECQTSP ARITHYQLNF QTGLLVPAHI FRIGPAPAFA
1160 1170 1180 1190 1200
GDTISLTITK GNEEGYFVTR RLNAYTGVVS LQRSVLEPRD FALDVEMKLW
1210 1220 1221
RQGSVTTFLA KMYIFFTTFA P

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3981 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fbln2
Peptide Sequence
PGFPVQEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
307
Peptide End
314
CPTAC ID
non-CPTAC-3981
Peptide Molecular Mass
900.4705
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 10.1 4.6 2.5 10.6 4.5 4.9 14.6 6.4 5.5 15 15 15
y3 (1+) 21.2 7.7 6.8 21.5 6.5 7.5 30.2 10.1 10.1 15 15 15
y5 (1+) 5.4 3.8 2.7 7.9 4.7 4.3 9.6 6 5.1 15 15 15
y6 (1+) 12.4 4.8 6.3 15 6.4 7.1 19.5 8 9.5 15 15 15
y7 (1+) 7.2 3.5 5.1 7.3 5 4.7 10.3 6.1 6.9 15 15 15
sum 3.6 1.5 1.9 6.7 2.7 3.2 7.6 3.1 3.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments