Rnh1, CPTAC-3997 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Rnh1	Rnh

Sequence Length (AA)	Molecular Weight (Da)
456	49816

Protein Name
Ribonuclease inhibitor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSLDIQCEQL	SDARWTELLP	LIQQYEVVRL	DDCGLTEVRC	KDISSAVQAN
60	70	80	90	100
PALTELSLRT	NELGDGGVGL	VLQGLQNPTC	KIQKLSLQNC	GLTEAGCGIL
110	120	130	140	150
PGMLRSLSTL	RELHLNDNPM	GDAGLKLLCE	GLQDPQCRLE	KLQLEYCNLT
160	170	180	190	200
ATSCEPLASV	LRVKADFKEL	VLSNNDLHEP	GVRILCQGLK	DSACQLESLK
210	220	230	240	250
LENCGITAAN	CKDLCDVVAS	KASLQELDLS	SNKLGNAGIA	ALCPGLLLPS
260	270	280	290	300
CKLRTLWLWE	CDITAEGCKD	LCRVLRAKQS	LKELSLASNE	LKDEGARLLC
310	320	330	340	350
ESLLEPGCQL	ESLWIKTCSL	TAASCPYFCS	VLTKSRSLLE	LQMSSNPLGD
360	370	380	390	400
EGVQELCKAL	SQPDTVLREL	WLGDCDVTNS	GCSSLANVLL	ANRSLRELDL
410	420	430	440	450
SNNCMGGPGV	LQLLESLKQP	SCTLQQLVLY	DIYWTNEVEE	QLRALEEERP
456
SLRIIS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3997 Collapse assay details

Data source: Panorama

Official Gene Symbol: Rnh1
Peptide Sequence: ALSQPDTVLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 359
Peptide End: 368

CPTAC ID: non-CPTAC-3997
Peptide Molecular Mass: 1,098.6033
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b4 (1+)	16.4	4.7	4.5	12.6	4.3	6.2	20.7	6.4	7.7	15	15	15
y8 (2+)	15.7	4.9	7.3	27.7	9.1	9.1	31.8	10.3	11.7	15	15	15
y4 (1+)	28.6	12.3	18	33.9	29.8	33.6	44.4	32.2	38.1	15	15	15
y6 (1+)	7.7	3.2	3.9	12	3.4	4.4	14.3	4.7	5.9	15	15	15
y8 (1+)	17.2	4.1	3.9	16.4	6.5	4.2	23.8	7.7	5.7	15	15	15
sum	6.5	3.6	4.4	10.8	10.1	10	12.6	10.7	10.9	15	15	15

Rnh1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3997 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Rnh1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3997 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments