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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Scube3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Scube3 N/A
Sequence Length (AA) Molecular Weight (Da)
993 108984
Protein Name
Signal peptide, CUB and EGF-like domain-containing protein 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGSGRVPGLC LLLLLVHARA AQHGKAAQDV DECVEGTDNC HIDAICQNTP
60 70 80 90 100
RSYKCICKSG YTGDGKHCKD VDECEREDNA GCVHDCVNIP GNYRCTCYDG
110 120 130 140 150
FHLAHDGHNC LDVDECAEGN GGCQQSCVNM MGSYECHCRD GFFLSDNQHT
160 170 180 190 200
CIQRPEEGMN CMNKNHGCAH ICRETPKGGI ACECRPGFEL TKNQRDCKLT
210 220 230 240 250
CNYGNGGCQH TCDDTEQGPR CGCHVKFVLH TDGKTCIETC AVNNGGCDSK
260 270 280 290 300
CHDAATGVHC SCPVGFMLQP DRKTCKDIDE CRLNNGGCDH ICRNTVGSFE
310 320 330 340 350
CSCKKGYKLL INERSCQDID ECSFDRTCDH MCVNTPGSFQ CLCHRGYLLY
360 370 380 390 400
GVTHCGDVDE CSINKGGCRF GCINTPGSYQ CTCPAGQGRL HWNGKDCTEP
410 420 430 440 450
VKCQSSLGAS KAMLSCNRSG KKDTCALTCP SRARFLPESE NGFTVSCGTP
460 470 480 490 500
SPKAAPARVI HSGNSTVSSS CHEAAVLPVK QRASFKIKDA KCRLHLRNKG
510 520 530 540 550
KAEEASRILG PGSVPCSDCL VTFIHLKCDS SRKGKGRRAR TPPGKEVTRL
560 570 580 590 600
TLELEAEVRA EETTAGCGLP CLRQRMERRL KGSLKMLRKS INQDRFLLRL
610 620 630 640 650
AGLDYELAHK PGLGAGDRAE LVEVCRPGQH RAGTKCVSCP QGTYYHGQTE
660 670 680 690 700
QCVPCPAGTF QEREGQLSCD LCPGSDAHGP LGATNVTTCA GQCPPGHHSG
710 720 730 740 750
DGFKPCQPCP RGTYQPEAGR TLCFPCGGGL TTKHEGAVSF QDCDTKVQCS
760 770 780 790 800
PGHYYNTSIH RCIRCAVGSY QPDFRQNFCT RCPGNTSTDF DGSTSVAQCK
810 820 830 840 850
NRQCGGELGE FTGYIESPNY PGNYPAGVEC VWNINPPPKR KILIVVPEIF
860 870 880 890 900
LPSEDECGDV LVMRKNSSPS SITTYETCQT YERPIAFTAR SRKLWINFKT
910 920 930 940 950
SEANSARGFQ IPYVTYDEDY EQLVEDIVRD GRLYASENHQ EILKDKKLIK
960 970 980 990 993
AFFEVLAHPQ NYFKYTEKHK EMLPKSFIKL LRSKVSSFLR PYK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4005 Collapse assay details

Data source: Panorama

Official Gene Symbol
Scube3
Peptide Sequence
AFFEVLAHPQNYFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
951
Peptide End
964
CPTAC ID
non-CPTAC-4005
Peptide Molecular Mass
1,709.8566
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.7 3 1.8 9.4 5.6 3.4 11 6.4 3.8 15 15 15
y12 (3+) 3.8 2.5 3.6 3.8 4.7 4.4 5.4 5.3 5.7 15 15 15
y10 (2+) 7 4 2.4 7.8 6.3 4.9 10.5 7.5 5.5 15 15 15
y11 (2+) 8.3 3.4 3.4 8.5 4 3.4 11.9 5.2 4.8 15 15 15
y12 (2+) 6.1 1.8 2.1 9.6 5 3.2 11.4 5.3 3.8 15 15 15
sum 4.5 1.2 1 7.1 4.5 2.8 8.4 4.7 3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments