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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Kndc1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Kndc1 Kiaa1768, Rasgef2, Vkind
Sequence Length (AA) Molecular Weight (Da)
1742 191313
Protein Name
Kinase non-catalytic C-lobe domain-containing protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQAMDPASRG FYEEDGKDLD FYDFEPLPTL PEDEENVSLA DILSLRDRGL
60 70 80 90 100
SEQEAWAVCL ECSLSMRSVA HAAIFQTLCI TPDTLAFNTS GNVCFMEQLS
110 120 130 140 150
DDPEGAFVPP EFDLTGNTFE AHIYSLGATL KAALEYVPEP ELEPKLSTDL
160 170 180 190 200
EGLLSQMQAE DPRERPDLAS IIALCEEKMQ PVSSCRLCRS LSAIGRRVLS
210 220 230 240 250
IESFGAFQEL SENTWRGRPA PRNVGPKKMP GDLSTDPEAL FPSKGLLQPP
260 270 280 290 300
ASRDAEQEAG QRPRAPSPKP LLSAPVRNGE NPGQEGLADL VLDARCPLGE
310 320 330 340 350
LDRDNLRRSR LKKAQTFPRL LQESTETSTL CLSLNGSRNQ LAISEFFPPD
360 370 380 390 400
PRKLFLEGKN GLSGFKTQSK SRLWPEQEPG VQLDKTPGAG RNPHRSPGAS
410 420 430 440 450
GQLEASSPSQ GSVEYKPSPS PVDAGDSDHE GHIPRSEEKI PEESRQPGST
460 470 480 490 500
ATEQSLSLKD LLSKLGRPFR EYELWALCLS CLSTLQTHKE HPAHLCLDNV
510 520 530 540 550
LVAEDGTVFF GPPPANGAYN SLFLAPEVSE EKLVTEKASV YCVAAVLWTA
560 570 580 590 600
AKFSVPRDHK LALPRRLKTL LLDMARRHAS ERPSAAEAIK VCSSYLLQRG
610 620 630 640 650
MDSSKILAHL RASTCKVHPE EETIGLQNAF SVVELKSTTA PAPESSPGFL
660 670 680 690 700
QVSNDTKLVA VPGPVPGLPP CCKEACELPA AFTSEATHFK PIVLAQDASV
710 720 730 740 750
TRDQLALPSE SNEKPKEGSG HLDREGTRKQ AALELVEATD LKMSNQLSPG
760 770 780 790 800
PELQGATPDP DGDSGSPSSA TECSCPHGPA LVTQQKGTSG TPSSPASSLP
810 820 830 840 850
PEHRPDGEGP LGTTVLPGPT SASQGSRHPC KPPRGKAAAS PSSPRGSDGH
860 870 880 890 900
PEKPRPADRK LCPSSVDTSS PPKMTACPSL QEAMRLIQEE FAFDGYMDNG
910 920 930 940 950
LEALIMGEYI YALKDLTFAT FCGAISEKFC DLYWDEQLLK NLFKVVNGPA
960 970 980 990 1000
SPSESTSEEP GSQPEHSPSR CSLSSKRPSL HGLGKEKPAT TWGSGGPCSP
1010 1020 1030 1040 1050
TALSDIDSDT LSQGNFEVGF RSQKSIKVTR EQQPEAEVGG QPGPSQDSTS
1060 1070 1080 1090 1100
HASDTVARLA RSEDGGPAGS PGASDFQNCS PGWSSAFYEA DCFGADVYNY
1110 1120 1130 1140 1150
VKDLERQKTN GHTELEAQSP ELEQQLMIEK RNYRKTLKFY QKLLQKEKRN
1160 1170 1180 1190 1200
KGSEVRTMLS KLRGQLDEMK SKVQFLSLVK KYLQVMYAER WGLEPCALPV
1210 1220 1230 1240 1250
IVNIAAAPCD TLDFSPLDES SSLIFYNVNK HPGSGRQKKA RILQAGTPLG
1260 1270 1280 1290 1300
LMAYLYSSDA FLEGYVQQFL YTFRYFCTPH DFLHFLLDRI SSTLSRAHQD
1310 1320 1330 1340 1350
PTSTFTKIYR RSLCVLQAWV EDCYTVDFIR NAGLLGQLED FISSKILPLD
1360 1370 1380 1390 1400
GTAEHLLALL EVGTERRADS ASRGADLEDP KEAEEDTRPF NALCKRFSED
1410 1420 1430 1440 1450
GITRKSFSWR LPRGNGLVLP HHKERQYTIA SALPKPCFFE DFYGPYAKAS
1460 1470 1480 1490 1500
EKGPYFLTEY STNQLFTQLT LLQQELFQKC HPVHFLNSRA LGVMDKSAAI
1510 1520 1530 1540 1550
PKASSSESLS AKTCSLFLPN YVQDKYLLQL LRNADDVSTW VAAEIVTSHT
1560 1570 1580 1590 1600
SKLQVNLLSK FLLIAKSCYE QRNFATAMQI LGGLEHLAVR QSPAWRILPA
1610 1620 1630 1640 1650
KIAEVMEELK AVEVFLKSDS LCLMEGRRFR AQPTLPSAHL LAMHIQQLET
1660 1670 1680 1690 1700
GGFTMTNGAH RWSKLRNIAK VASQVHAFQE NPYTFSPDPK LQAHLKQRIA
1710 1720 1730 1740 1742
RFSGADVSIL AADNRANFHQ IPGEKHSRKI QDKLRRMKAT FQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4014 Collapse assay details

Data source: Panorama

Official Gene Symbol
Kndc1
Peptide Sequence
VYNYVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1097
Peptide End
1102
CPTAC ID
non-CPTAC-4014
Peptide Molecular Mass
784.4119
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 6.1 3.7 4.9 8.1 7.4 6.1 10.1 8.3 7.8 15 15 15
y5 (1+) 8 4.7 5.9 9.4 4.9 5.4 12.3 6.8 8 15 15 15
b3 (1+) 28.2 7 7.8 30.8 10.6 7.3 41.8 12.7 10.7 15 15 15
b2 (1+) 10.5 7.5 4.9 11.6 8.4 7.5 15.6 11.3 9 15 15 15
y2 (1+) 22.1 8.5 5.3 22.9 23.1 17.4 31.8 24.6 18.2 15 15 15
sum 4.6 2.1 3.2 6.3 4.4 4.6 7.8 4.9 5.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments