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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cntn4 Kiaa3024
Sequence Length (AA) Molecular Weight (Da)
1026 113490
Protein Name
Contactin-4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLPWELLVL QSFMLCLADD YTLHGPVFVQ EPSHVMFPLD SEEKKVKLSC
60 70 80 90 100
EVKGNPKPHI RWKINGTDVD IGMDFRYSVV DGSLLINNPN KTQDAGTYQC
110 120 130 140 150
IATNSFGTIV SREAKLQFAY LENFKTRTRS TVSVRRGQGM VLLCGPPPHS
160 170 180 190 200
GELSYAWIFN EYPSYQDNRR FVSQETGNLY IAKVEKSDVG NYTCVVTNTV
210 220 230 240 250
TNHKVLGPPT PLILRNDGVM GEYEPKIEVQ FPETVPAEKG TTVKLECFAL
260 270 280 290 300
GNPVPTILWR RADGKPIARK ARRHKSNGIL EIPNFQQEDA GSYECVAENS
310 320 330 340 350
RGKNVAKGQL TFYAQPNWVQ IINDIHVAME ESVFWECKAN GRPKPTYRWL
360 370 380 390 400
KNGDPLLTRD RIQIEQGTLN ITIVNLSDAG MYQCVAENKH GVIFSSAELS
410 420 430 440 450
VIAESPDFSR TLLKRVTLVK VGGEVVIECK PKASPRPVYT WRKGREILRE
460 470 480 490 500
NERITISEDG NLRIINVTKS DAGSYTCIAT NHFGTASSTG NVIVKDPTKV
510 520 530 540 550
MVPPSSMDVT VGESIVLPCQ VTHDHSLDIV FTWTFNGHLI DFDKDGDHFE
560 570 580 590 600
RVGGQDSAGD LMIRNIQLKH AGKYVCMVQT SVDKLSVAAD LIVRGPPGPP
610 620 630 640 650
EAVTIDEITD TTAQLSWRPG PDNHSPITMY VIQARTPFSV GWQAVNTVPD
660 670 680 690 700
LVDGKTFTAT VVGLNPWVEY EFRTVAANVI GIGEPSRPSE KRRTEEALPE
710 720 730 740 750
VTPANVSGGG GSKSELVITW ETVPEELQNG RGFGYVVAFR PHGKMIWMLT
760 770 780 790 800
VLASADASRY VFRNESVRPF SPFEVKVGVF NNKGEGPFSP TTLVYSAEEE
810 820 830 840 850
PTKPPASIFA RSLSATDIEV FWASPIGKNR GRIQGYEVKY WRHDDKEENA
860 870 880 890 900
KKIRTVGNQT STKITNLKGS ALYHLSVKAY NSAGTGPSSA TVNVTTRKPP
910 920 930 940 950
PSQPPGNIIW NSSDSKIILN WDQVKALDNE SEVKGYKVLY RWNRQSSTSV
960 970 980 990 1000
IETNKTSVEL SLPFDEDYII EIKPFSDGGD GSSSEQIRIP KISNSYARGS
1010 1020 1026
GASTSNACTL SAISTIMISL TARSSL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4027 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cntn4
Peptide Sequence
IEVQFPETVPAEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
227
Peptide End
239
CPTAC ID
non-CPTAC-4027
Peptide Molecular Mass
1,485.7715
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 10.5 7.1 3.6 12.3 10.8 8.3 16.2 12.9 9 15 15 15
y8 (1+) 9.4 3.7 3.1 12.6 7.1 5.3 15.7 8 6.1 15 15 15
y4 (1+) 10.2 5.5 3.1 10.3 5.6 5.5 14.5 7.8 6.3 15 15 15
b2 (1+) 6.1 5.5 3.2 11.1 7.9 3 12.7 9.6 4.4 15 15 15
b3 (1+) 15.1 8.1 5.3 15.9 6.8 6.2 21.9 10.6 8.2 15 15 15
sum 3.5 3.1 1.5 8.1 6 3.9 8.8 6.8 4.2 15 15 15


Additional Resources and Comments