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Overview Data source: UniProt

Official Gene Symbol Other Aliases
FGR SRC2
Sequence Length (AA) Molecular Weight (Da)
529 59479
Protein Name
Tyrosine-protein kinase Fgr
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGCVFCKKLE PVATAKEDAG LEGDFRSYGA ADHYGPDPTK ARPASSFAHI
60 70 80 90 100
PNYSNFSSQA INPGFLDSGT IRGVSGIGVT LFIALYDYEA RTEDDLTFTK
110 120 130 140 150
GEKFHILNNT EGDWWEARSL SSGKTGCIPS NYVAPVDSIQ AEEWYFGKIG
160 170 180 190 200
RKDAERQLLS PGNPQGAFLI RESETTKGAY SLSIRDWDQT RGDHVKHYKI
210 220 230 240 250
RKLDMGGYYI TTRVQFNSVQ ELVQHYMEVN DGLCNLLIAP CTIMKPQTLG
260 270 280 290 300
LAKDAWEISR SSITLERRLG TGCFGDVWLG TWNGSTKVAV KTLKPGTMSP
310 320 330 340 350
KAFLEEAQVM KLLRHDKLVQ LYAVVSEEPI YIVTEFMCHG SLLDFLKNPE
360 370 380 390 400
GQDLRLPQLV DMAAQVAEGM AYMERMNYIH RDLRAANILV GERLACKIAD
410 420 430 440 450
FGLARLIKDD EYNPCQGSKF PIKWTAPEAA LFGRFTIKSD VWSFGILLTE
460 470 480 490 500
LITKGRIPYP GMNKREVLEQ VEQGYHMPCP PGCPASLYEA MEQTWRLDPE
510 520 529
ERPTFEYLQS FLEDYFTSAE PQYQPGDQT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1786 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGR
Peptide Modified Sequence
GAY[+80.0]SLSIR
Modification Type
Phospho (Y)
Protein - Site of Modification
180
Peptide - Site of Modification
3
Peptide Start
178
Peptide End
185
CPTAC ID
CPTAC-1786
Peptide Molecular Mass
945.4321
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 8.3 3.1 2.8 8.2 5.6 3.4 11.7 6.4 4.4 15 15 15
y3 (1+) 8.7 3.7 4.3 8.4 4.7 4.9 12.1 6 6.5 14 15 15
y5 (1+) 14.4 4.9 2.2 18.9 5.2 2.8 23.8 7.1 3.6 15 15 15
y4 (1+) 7.9 7.6 3.9 10.5 7.4 4.9 13.1 10.6 6.3 14 15 15
sum 10.3 3.6 2.2 11.9 4.5 3.1 15.7 5.8 3.8 15 15 15

Additional Resources and Comments