FGR, CPTAC-3064 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FGR	SRC2

Sequence Length (AA)	Molecular Weight (Da)
529	59479

Protein Name
Tyrosine-protein kinase Fgr

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGCVFCKKLE	PVATAKEDAG	LEGDFRSYGA	ADHYGPDPTK	ARPASSFAHI
60	70	80	90	100
PNYSNFSSQA	INPGFLDSGT	IRGVSGIGVT	LFIALYDYEA	RTEDDLTFTK
110	120	130	140	150
GEKFHILNNT	EGDWWEARSL	SSGKTGCIPS	NYVAPVDSIQ	AEEWYFGKIG
160	170	180	190	200
RKDAERQLLS	PGNPQGAFLI	RESETTKGAY	SLSIRDWDQT	RGDHVKHYKI
210	220	230	240	250
RKLDMGGYYI	TTRVQFNSVQ	ELVQHYMEVN	DGLCNLLIAP	CTIMKPQTLG
260	270	280	290	300
LAKDAWEISR	SSITLERRLG	TGCFGDVWLG	TWNGSTKVAV	KTLKPGTMSP
310	320	330	340	350
KAFLEEAQVM	KLLRHDKLVQ	LYAVVSEEPI	YIVTEFMCHG	SLLDFLKNPE
360	370	380	390	400
GQDLRLPQLV	DMAAQVAEGM	AYMERMNYIH	RDLRAANILV	GERLACKIAD
410	420	430	440	450
FGLARLIKDD	EYNPCQGSKF	PIKWTAPEAA	LFGRFTIKSD	VWSFGILLTE
460	470	480	490	500
LITKGRIPYP	GMNKREVLEQ	VEQGYHMPCP	PGCPASLYEA	MEQTWRLDPE
510	520	529
ERPTFEYLQS	FLEDYFTSAE	PQYQPGDQT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1786 Collapse assay details

Data source: Panorama

Official Gene Symbol: FGR
Peptide Modified Sequence: GAY[+80.0]SLSIR
Modification Type: Phospho (Y)
Protein - Site of Modification: 180
Peptide - Site of Modification: 3
Peptide Start: 178
Peptide End: 185

CPTAC ID: CPTAC-1786
Peptide Molecular Mass: 945.4321
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: E.coli lysate
Submitting Laboratory: Johns Hopkins University / Pandey
Submitting Lab PI: Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Fusion Lumos
Internal Standard: 100fmol
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy nLC 1200 System
Column Packing: C18 2um 100A
Column Dimensions: 75um i.d. x 50cm
Flow Rate: 300 nl/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	8.3	3.1	2.8	8.2	5.6	3.4	11.7	6.4	4.4	15	15	15
y3 (1+)	8.7	3.7	4.3	8.4	4.7	4.9	12.1	6	6.5	14	15	15
y5 (1+)	14.4	4.9	2.2	18.9	5.2	2.8	23.8	7.1	3.6	15	15	15
y4 (1+)	7.9	7.6	3.9	10.5	7.4	4.9	13.1	10.6	6.3	14	15	15
sum	10.3	3.6	2.2	11.9	4.5	3.1	15.7	5.8	3.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3064 Collapse assay details

Data source: Panorama

Official Gene Symbol: FGR
Peptide Sequence: QLLSPGNPQGAFLIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 157
Peptide End: 171

CPTAC ID: CPTAC-3064
Peptide Molecular Mass: 1,609.8940
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend