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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TNK2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TNK2 ACK1
Sequence Length (AA) Molecular Weight (Da)
1038 114569
Protein Name
Activated CDC42 kinase 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQPEEGTGWL LELLSEVQLQ QYFLRLRDDL NVTRLSHFEY VKNEDLEKIG
60 70 80 90 100
MGRPGQRRLW EAVKRRKALC KRKSWMSKVF SGKRLEAEFP PHHSQSTFRK
110 120 130 140 150
TSPAPGGPAG EGPLQSLTCL IGEKDLRLLE KLGDGSFGVV RRGEWDAPSG
160 170 180 190 200
KTVSVAVKCL KPDVLSQPEA MDDFIREVNA MHSLDHRNLI RLYGVVLTPP
210 220 230 240 250
MKMVTELAPL GSLLDRLRKH QGHFLLGTLS RYAVQVAEGM GYLESKRFIH
260 270 280 290 300
RDLAARNLLL ATRDLVKIGD FGLMRALPQN DDHYVMQEHR KVPFAWCAPE
310 320 330 340 350
SLKTRTFSHA SDTWMFGVTL WEMFTYGQEP WIGLNGSQIL HKIDKEGERL
360 370 380 390 400
PRPEDCPQDI YNVMVQCWAH KPEDRPTFVA LRDFLLEAQP TDMRALQDFE
410 420 430 440 450
EPDKLHIQMN DVITVIEGRA ENYWWRGQNT RTLCVGPFPR NVVTSVAGLS
460 470 480 490 500
AQDISQPLQN SFIHTGHGDS DPRHCWGFPD RIDELYLGNP MDPPDLLSVE
510 520 530 540 550
LSTSRPPQHL GGVKKPTYDP VSEDQDPLSS DFKRLGLRKP GLPRGLWLAK
560 570 580 590 600
PSARVPGTKA SRGSGAEVTL IDFGEEPVVP ALRPCAPSLA QLAMDACSLL
610 620 630 640 650
DETPPQSPTR ALPRPLHPTP VVDWDARPLP PPPAYDDVAQ DEDDFEICSI
660 670 680 690 700
NSTLVGAGVP AGPSQGQTNY AFVPEQARPP PPLEDNLFLP PQGGGKPPSS
710 720 730 740 750
AQTAEIFQAL QQECMRQLQA PAGSPAPSPS PGGDDKPQVP PRVPIPPRPT
760 770 780 790 800
RPHVQLSPAP PGEEETSQWP GPASPPRVPP REPLSPQGSR TPSPLVPPGS
810 820 830 840 850
SPLPPRLSSS PGKTMPTTQS FASDPKYATP QVIQAPGPRA GPCILPIVRD
860 870 880 890 900
GKKVSSTHYY LLPERPSYLE RYQRFLREAQ SPEEPTPLPV PLLLPPPSTP
910 920 930 940 950
APAAPTATVR PMPQAALDPK ANFSTNNSNP GARPPPPRAT ARLPQRGCPG
960 970 980 990 1000
DGPEAGRPAD KIQMAMVHGV TTEECQAALQ CHGWSVQRAA QYLKVEQLFG
1010 1020 1030 1038
LGLRPRGECH KVLEMFDWNL EQAGCHLLGS WGPAHHKR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1779 Collapse assay details

Data source: Panorama

Official Gene Symbol
TNK2
Peptide Modified Sequence
ALPQNDDHY[+80.0]VMQEHR
Modification Type
Phospho (Y)
Protein - Site of Modification
284
Peptide - Site of Modification
9
Peptide Start
276
Peptide End
290
CPTAC ID
CPTAC-1779
Peptide Molecular Mass
1,931.7986
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 71.2 19 13 77 24.3 12.8 104.9 30.8 18.2 15 15 15
y8 (1+) 38.8 12 9.1 46.3 13.2 8.3 60.4 17.8 12.3 15 15 15
y7 (1+) 85.5 10.9 6.8 127.7 16.1 7.6 153.7 19.4 10.2 15 15 15
y6 (1+) 84.1 11 5.5 91.9 18.5 6.7 124.6 21.5 8.7 15 15 15
y5 (1+) 62.7 7.2 7.5 62.8 10.5 6.9 88.7 12.7 10.2 15 15 15
sum 29.1 9 6 48.9 12 5.2 56.9 15 7.9 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Assay Details for CPTAC-2789 Collapse assay details

Data source: Panorama

Official Gene Symbol
TNK2
Peptide Modified Sequence
Y[+80.0]ATPQVIQAPGPR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
1
Peptide Start
827
Peptide End
839
CPTAC ID
CPTAC-2789
Peptide Molecular Mass
1,476.7126
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6.7 4.5 5.4 14.5 7.4 5.1 16 8.7 7.4 15 15 15
y8 (1+) 6.6 4.2 5.8 14.3 6.6 5.8 15.7 7.8 8.2 15 15 15
y7 (1+) 5.5 4.4 5.4 14.1 6.2 5.4 15.1 7.6 7.6 15 15 15
y6 (1+) 5.9 3 5.4 13.5 5.8 5.2 14.7 6.5 7.5 15 15 15
sum 6 4 5.4 14.1 6.5 5.3 15.3 7.6 7.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments