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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PLK1 PLK
Sequence Length (AA) Molecular Weight (Da)
603 68255
Protein Name
Serine/threonine-protein kinase PLK1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSAAVTAGKL ARAPADPGKA GVPGVAAPGA PAAAPPAKEI PEVLVDPRSR
60 70 80 90 100
RRYVRGRFLG KGGFAKCFEI SDADTKEVFA GKIVPKSLLL KPHQREKMSM
110 120 130 140 150
EISIHRSLAH QHVVGFHGFF EDNDFVFVVL ELCRRRSLLE LHKRRKALTE
160 170 180 190 200
PEARYYLRQI VLGCQYLHRN RVIHRDLKLG NLFLNEDLEV KIGDFGLATK
210 220 230 240 250
VEYDGERKKT LCGTPNYIAP EVLSKKGHSF EVDVWSIGCI MYTLLVGKPP
260 270 280 290 300
FETSCLKETY LRIKKNEYSI PKHINPVAAS LIQKMLQTDP TARPTINELL
310 320 330 340 350
NDEFFTSGYI PARLPITCLT IPPRFSIAPS SLDPSNRKPL TVLNKGLENP
360 370 380 390 400
LPERPREKEE PVVRETGEVV DCHLSDMLQQ LHSVNASKPS ERGLVRQEEA
410 420 430 440 450
EDPACIPIFW VSKWVDYSDK YGLGYQLCDN SVGVLFNDST RLILYNDGDS
460 470 480 490 500
LQYIERDGTE SYLTVSSHPN SLMKKITLLK YFRNYMSEHL LKAGANITPR
510 520 530 540 550
EGDELARLPY LRTWFRTRSA IILHLSNGSV QINFFQDHTK LILCPLMAAV
560 570 580 590 600
TYIDEKRDFR TYRLSLLEEY GCCKELASRL RYARTMVDKL LSSRSASNRL
603
KAS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1327 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLK1
Peptide Modified Sequence
KT[+80.0]LC[+57.0]GTPNYIAPEVLSK
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
210, 212
Peptide - Site of Modification
2, 4
Peptide Start
209
Peptide End
225
CPTAC ID
CPTAC-1327
Peptide Molecular Mass
1,969.9584
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.3 4.7 3.7 7.6 5.6 7.3 9.9 7.3 8.2 15 15 15
y6 (1+) 5.9 3.6 3.9 6.7 5.7 9.7 8.9 6.7 10.5 15 15 15
b8 (1+) 12.8 11.1 9 13.5 16.4 11.6 18.6 19.8 14.7 15 15 15
sum 4.1 3.5 3 5.3 4.8 7.9 6.7 5.9 8.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1326 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLK1
Peptide Modified Sequence
RS[+80.0]LLELHK
Modification Type
Phospho (ST)
Protein - Site of Modification
137
Peptide - Site of Modification
2
Peptide Start
136
Peptide End
143
CPTAC ID
CPTAC-1326
Peptide Molecular Mass
1,074.5587
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 17 12.4 10.7 20 14.1 12.6 26.2 18.8 16.5 15 15 15
b3-98 (1+) 15.1 4.8 3.4 10.7 4.9 12.7 18.5 6.9 13.1 15 15 15
b4-98 (1+) 18.4 16.1 6.9 21.7 18.1 10.3 28.5 24.2 12.4 15 15 15
sum 15.1 4 3.6 10.7 4.2 11.3 18.5 5.8 11.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1264 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLK1
Peptide Sequence
HINPVAASLIQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
273
Peptide End
284
CPTAC ID
CPTAC-1264
Peptide Molecular Mass
1,289.7456
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 35.2 16.2 16.5 59.6 15.2 12.7 69.2 22.2 20.8 15 15 15
y5 (1+) 41.4 23.8 15.2 50.3 30.4 13.4 65.1 38.6 20.3 15 15 15
y7 (1+) 25.1 11.6 12.3 33.7 13.3 13.4 42 17.6 18.2 15 15 15
sum 25.4 9.6 9.8 41.6 10.4 9.8 48.7 14.2 13.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2909 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLK1
Peptide Sequence
ALTEPEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
147
Peptide End
154
CPTAC ID
CPTAC-2909
Peptide Molecular Mass
885.4556
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.1 1 3.9 5.6 1.9 5.3 7.6 2.1 6.6 15 15 15
y4 (1+) 4.1 1.4 3.2 4.8 1.6 3.3 6.3 2.1 4.6 15 15 15
b3 (1+) 9.6 3.8 4.7 8.4 3.6 5.8 12.8 5.2 7.5 15 15 15
sum 3.4 1.2 3.2 4.3 1.4 3.4 5.5 1.8 4.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2908 Collapse assay details

Data source: Panorama

Official Gene Symbol
PLK1
Peptide Sequence
LGNLFLNEDLEVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
179
Peptide End
191
CPTAC ID
CPTAC-2908
Peptide Molecular Mass
1,502.7981
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 5.6 3.4 8 11.7 10.4 14.2 13 10.9 16.3 15 15 15
b3 (1+) 9 2.8 5.7 13.9 7.8 10.4 16.6 8.3 11.9 15 15 15
y8 (1+) 10.8 2.8 7.2 15.4 10.5 13.5 18.8 10.9 15.3 15 15 15
sum 7.9 2.8 6.5 12.7 9 12 15 9.4 13.6 15 15 15


Additional Resources and Comments