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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ABL1 ABL, JTK7
Sequence Length (AA) Molecular Weight (Da)
1130 122873
Protein Name
Tyrosine-protein kinase ABL1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLEICLKLVG CKSKKGLSSS SSCYLEEALQ RPVASDFEPQ GLSEAARWNS
60 70 80 90 100
KENLLAGPSE NDPNLFVALY DFVASGDNTL SITKGEKLRV LGYNHNGEWC
110 120 130 140 150
EAQTKNGQGW VPSNYITPVN SLEKHSWYHG PVSRNAAEYL LSSGINGSFL
160 170 180 190 200
VRESESSPGQ RSISLRYEGR VYHYRINTAS DGKLYVSSES RFNTLAELVH
210 220 230 240 250
HHSTVADGLI TTLHYPAPKR NKPTVYGVSP NYDKWEMERT DITMKHKLGG
260 270 280 290 300
GQYGEVYEGV WKKYSLTVAV KTLKEDTMEV EEFLKEAAVM KEIKHPNLVQ
310 320 330 340 350
LLGVCTREPP FYIITEFMTY GNLLDYLREC NRQEVNAVVL LYMATQISSA
360 370 380 390 400
MEYLEKKNFI HRDLAARNCL VGENHLVKVA DFGLSRLMTG DTYTAHAGAK
410 420 430 440 450
FPIKWTAPES LAYNKFSIKS DVWAFGVLLW EIATYGMSPY PGIDLSQVYE
460 470 480 490 500
LLEKDYRMER PEGCPEKVYE LMRACWQWNP SDRPSFAEIH QAFETMFQES
510 520 530 540 550
SISDEVEKEL GKQGVRGAVS TLLQAPELPT KTRTSRRAAE HRDTTDVPEM
560 570 580 590 600
PHSKGQGESD PLDHEPAVSP LLPRKERGPP EGGLNEDERL LPKDKKTNLF
610 620 630 640 650
SALIKKKKKT APTPPKRSSS FREMDGQPER RGAGEEEGRD ISNGALAFTP
660 670 680 690 700
LDTADPAKSP KPSNGAGVPN GALRESGGSG FRSPHLWKKS STLTSSRLAT
710 720 730 740 750
GEEEGGGSSS KRFLRSCSAS CVPHGAKDTE WRSVTLPRDL QSTGRQFDSS
760 770 780 790 800
TFGGHKSEKP ALPRKRAGEN RSDQVTRGTV TPPPRLVKKN EEAADEVFKD
810 820 830 840 850
IMESSPGSSP PNLTPKPLRR QVTVAPASGL PHKEEAGKGS ALGTPAAAEP
860 870 880 890 900
VTPTSKAGSG APGGTSKGPA EESRVRRHKH SSESPGRDKG KLSRLKPAPP
910 920 930 940 950
PPPAASAGKA GGKPSQSPSQ EAAGEAVLGA KTKATSLVDA VNSDAAKPSQ
960 970 980 990 1000
PGEGLKKPVL PATPKPQSAK PSGTPISPAP VPSTLPSASS ALAGDQPSST
1010 1020 1030 1040 1050
AFIPLISTRV SLRKTRQPPE RIASGAITKG VVLDSTEALC LAISRNSEQM
1060 1070 1080 1090 1100
ASHSAVLEAG KNLYTFCVSY VDSIQQMRNK FAFREAINKL ENNLRELQIC
1110 1120 1130
PATAGSGPAA TQDFSKLLSS VKEISDIVQR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1776 Collapse assay details

Data source: Panorama

Official Gene Symbol
ABL1
Peptide Modified Sequence
LMTGDTY[+80.0]TAHAGAK
Modification Type
Phospho (Y)
Protein - Site of Modification
393
Peptide - Site of Modification
7
Peptide Start
387
Peptide End
400
CPTAC ID
CPTAC-1776
Peptide Molecular Mass
1,515.6429
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (1+) 4.8 5.5 4.5 7.4 6.5 5.7 8.8 8.5 7.3 15 15 15
y11 (1+) 4.8 7.7 4.2 8.1 7.5 5.7 9.4 10.7 7.1 15 15 15
y9 (1+) 6.2 8.3 2.7 8 8.2 5.4 10.1 11.7 6 15 15 15
y7 (1+) 6.3 7.6 4.5 7.9 7.8 7 10.1 10.9 8.3 15 15 15
y5 (1+) 5.5 9.9 4.2 5.8 9.6 7.7 8 13.8 8.8 15 15 15
b2 (1+) 5.3 7 3.7 7.4 5.4 6.6 9.1 8.8 7.6 15 15 15
sum 4.2 6.8 3.1 6.3 6.7 5.5 7.6 9.5 6.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1788 Collapse assay details

Data source: Panorama

Official Gene Symbol
ABL1
Peptide Modified Sequence
LY[+80.0]VSSESR
Modification Type
Phospho (Y)
Protein - Site of Modification
185
Peptide - Site of Modification
2
Peptide Start
184
Peptide End
191
CPTAC ID
CPTAC-1788
Peptide Molecular Mass
1,019.4325
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) NA 44.5 24.3 NA 48.5 29.3 NA 65.8 38.1 2 15 15
b2 (1+) 16.3 8.2 8 11.4 9.9 8.7 19.9 12.9 11.8 15 15 15
y2 (1+) 96.4 16.7 10.2 8.7 19.9 9.2 96.8 26 13.7 5 15 15
y3 (1+) NA 0.5 34.8 NA 25 24.2 NA 25 42.4 1 5 10
y4 (1+) NA 47.2 17.5 NA 59.4 19.5 NA 75.9 26.2 2 15 15
y5 (1+) 28.4 13 8.2 27.7 14.3 8.2 39.7 19.3 11.6 15 15 15
y6 (1+) 10.8 9.1 5.8 10.8 8.6 7.1 15.3 12.5 9.2 15 15 15
sum 12.6 7.1 5 12.5 7.8 5.4 17.7 10.5 7.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3042 Collapse assay details

Data source: Panorama

Official Gene Symbol
ABL1
Peptide Sequence
ATSLVDAVNSDAAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
934
Peptide End
947
CPTAC ID
CPTAC-3042
Peptide Molecular Mass
1,360.6834
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 5.7 5.5 4.4 6.9 4.6 3.9 8.9 7.2 5.9 15 15 15
y9 (1+) 5.4 5.4 2.5 7.5 4.8 2 9.2 7.2 3.2 15 15 15
y6 (1+) 7.3 4.9 3.9 9.8 4.2 3.5 12.2 6.5 5.2 15 15 15
sum 5.3 5.2 3.2 7.4 4.4 2.7 9.1 6.8 4.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3041 Collapse assay details

Data source: Panorama

Official Gene Symbol
ABL1
Peptide Sequence
LYVSSESR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
184
Peptide End
191
CPTAC ID
CPTAC-3041
Peptide Molecular Mass
939.4662
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 4.5 2.4 3.2 4.5 1.8 3.4 6.4 3 4.7 15 15 15
y4 (1+) 6.1 3.6 4.6 6.5 3.6 4.2 8.9 5.1 6.2 15 15 15
y6 (1+) 2.7 2 2.9 3.5 1.8 2.9 4.4 2.7 4.1 15 15 15
sum 2.4 1.8 2.9 2.9 1.3 2.9 3.8 2.2 4.1 15 15 15


Additional Resources and Comments