SYK, CPTAC-3143 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SYK	N/A

Sequence Length (AA)	Molecular Weight (Da)
635	72066

Protein Name
Tyrosine-protein kinase SYK

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MASSGMADSA	NHLPFFFGNI	TREEAEDYLV	QGGMSDGLYL	LRQSRNYLGG
60	70	80	90	100
FALSVAHGRK	AHHYTIEREL	NGTYAIAGGR	THASPADLCH	YHSQESDGLV
110	120	130	140	150
CLLKKPFNRP	QGVQPKTGPF	EDLKENLIRE	YVKQTWNLQG	QALEQAIISQ
160	170	180	190	200
KPQLEKLIAT	TAHEKMPWFH	GKISREESEQ	IVLIGSKTNG	KFLIRARDNN
210	220	230	240	250
GSYALCLLHE	GKVLHYRIDK	DKTGKLSIPE	GKKFDTLWQL	VEHYSYKADG
260	270	280	290	300
LLRVLTVPCQ	KIGTQGNVNF	GGRPQLPGSH	PATWSAGGII	SRIKSYSFPK
310	320	330	340	350
PGHRKSSPAQ	GNRQESTVSF	NPYEPELAPW	AADKGPQREA	LPMDTEVYES
360	370	380	390	400
PYADPEEIRP	KEVYLDRKLL	TLEDKELGSG	NFGTVKKGYY	QMKKVVKTVA
410	420	430	440	450
VKILKNEAND	PALKDELLAE	ANVMQQLDNP	YIVRMIGICE	AESWMLVMEM
460	470	480	490	500
AELGPLNKYL	QQNRHVKDKN	IIELVHQVSM	GMKYLEESNF	VHRDLAARNV
510	520	530	540	550
LLVTQHYAKI	SDFGLSKALR	ADENYYKAQT	HGKWPVKWYA	PECINYYKFS
560	570	580	590	600
SKSDVWSFGV	LMWEAFSYGQ	KPYRGMKGSE	VTAMLEKGER	MGCPAGCPRE
610	620	630	635
MYDLMNLCWT	YDVENRPGFA	AVELRLRNYY	YDVVN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1174 Collapse assay details

Data source: Panorama

Official Gene Symbol: SYK
Peptide Sequence: LIATTAHEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 157
Peptide End: 165

CPTAC ID: CPTAC-1174
Peptide Molecular Mass: 982.5447
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	26.1	28.5	15.1	28.4	32.1	16	38.6	42.9	22	15	15	15
y5 (1+)	17	9	10.3	15.2	12.2	12.9	22.8	15.2	16.5	15	15	15
y6 (1+)	13.8	12.3	14.3	18.1	13.4	13	22.8	18.2	19.3	15	15	15
sum	11.4	7.1	5.6	13.2	8.8	7.2	17.4	11.3	9.1	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-1173 Collapse assay details

Data source: Panorama

Official Gene Symbol: SYK
Peptide Sequence: NYLGGFALSVAHGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 46
Peptide End: 59

CPTAC ID: CPTAC-1173
Peptide Molecular Mass: 1,460.7524
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu