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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SYK N/A
Sequence Length (AA) Molecular Weight (Da)
635 72066
Protein Name
Tyrosine-protein kinase SYK
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASSGMADSA NHLPFFFGNI TREEAEDYLV QGGMSDGLYL LRQSRNYLGG
60 70 80 90 100
FALSVAHGRK AHHYTIEREL NGTYAIAGGR THASPADLCH YHSQESDGLV
110 120 130 140 150
CLLKKPFNRP QGVQPKTGPF EDLKENLIRE YVKQTWNLQG QALEQAIISQ
160 170 180 190 200
KPQLEKLIAT TAHEKMPWFH GKISREESEQ IVLIGSKTNG KFLIRARDNN
210 220 230 240 250
GSYALCLLHE GKVLHYRIDK DKTGKLSIPE GKKFDTLWQL VEHYSYKADG
260 270 280 290 300
LLRVLTVPCQ KIGTQGNVNF GGRPQLPGSH PATWSAGGII SRIKSYSFPK
310 320 330 340 350
PGHRKSSPAQ GNRQESTVSF NPYEPELAPW AADKGPQREA LPMDTEVYES
360 370 380 390 400
PYADPEEIRP KEVYLDRKLL TLEDKELGSG NFGTVKKGYY QMKKVVKTVA
410 420 430 440 450
VKILKNEAND PALKDELLAE ANVMQQLDNP YIVRMIGICE AESWMLVMEM
460 470 480 490 500
AELGPLNKYL QQNRHVKDKN IIELVHQVSM GMKYLEESNF VHRDLAARNV
510 520 530 540 550
LLVTQHYAKI SDFGLSKALR ADENYYKAQT HGKWPVKWYA PECINYYKFS
560 570 580 590 600
SKSDVWSFGV LMWEAFSYGQ KPYRGMKGSE VTAMLEKGER MGCPAGCPRE
610 620 630 635
MYDLMNLCWT YDVENRPGFA AVELRLRNYY YDVVN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1174 Collapse assay details

Data source: Panorama

Official Gene Symbol
SYK
Peptide Sequence
LIATTAHEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
157
Peptide End
165
CPTAC ID
CPTAC-1174
Peptide Molecular Mass
982.5447
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 26.1 28.5 15.1 28.4 32.1 16 38.6 42.9 22 15 15 15
y5 (1+) 17 9 10.3 15.2 12.2 12.9 22.8 15.2 16.5 15 15 15
y6 (1+) 13.8 12.3 14.3 18.1 13.4 13 22.8 18.2 19.3 15 15 15
sum 11.4 7.1 5.6 13.2 8.8 7.2 17.4 11.3 9.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1173 Collapse assay details

Data source: Panorama

Official Gene Symbol
SYK
Peptide Sequence
NYLGGFALSVAHGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
46
Peptide End
59
CPTAC ID
CPTAC-1173
Peptide Molecular Mass
1,460.7524
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 20.5 5.7 10 20.9 10 10.7 29.3 11.5 14.6 15 15 15
y6 (1+) 11.3 3.5 7 10.3 7.4 7.2 15.3 8.2 10 15 15 15
y7 (1+) 18.4 7.8 4.7 18.2 13.2 4.6 25.9 15.3 6.6 15 15 15
sum 12.2 3.2 4.9 8.7 8.2 5.4 15 8.8 7.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3143 Collapse assay details

Data source: Panorama

Official Gene Symbol
SYK
Peptide Sequence
NVLLVTQHYAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
499
Peptide End
509
CPTAC ID
CPTAC-3143
Peptide Molecular Mass
1,284.7190
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.7 7.7 3.8 8.3 8.5 3.7 10.7 11.5 5.3 15 15 15
y6 (1+) 4.5 5.7 3.7 6.1 6.6 3.7 7.6 8.7 5.2 15 15 15
y4 (1+) 7.1 7.1 3.8 13 7.4 4 14.8 10.3 5.5 15 15 15
sum 4.5 6.4 3.4 7 7.2 3.3 8.3 9.6 4.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3142 Collapse assay details

Data source: Panorama

Official Gene Symbol
SYK
Peptide Modified Sequence
WYAPEC[+57.0]INYYK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
544
Peptide - Site of Modification
6
Peptide Start
538
Peptide End
548
CPTAC ID
CPTAC-3142
Peptide Molecular Mass
1,505.6649
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 5 5.3 3.9 5.7 6.7 4.2 7.6 8.5 5.7 15 15 15
y8 (1+) 4.9 5.1 3.9 5.3 6.3 4.2 7.2 8.1 5.7 15 15 15
y4 (1+) 6.4 4.5 2.8 6.8 5.1 3.2 9.3 6.8 4.3 15 15 15
sum 4.8 4.9 3.7 5.2 6.1 4 7.1 7.8 5.4 15 15 15


Additional Resources and Comments