FRK, CPTAC-3141 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FRK	PTK5, RAK

Sequence Length (AA)	Molecular Weight (Da)
505	58254

Protein Name
Tyrosine-protein kinase FRK

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSNICQRLWE	YLEPYLPCLS	TEADKSTVIE	NPGALCSPQS	QRHGHYFVAL
60	70	80	90	100
FDYQARTAED	LSFRAGDKLQ	VLDTLHEGWW	FARHLEKRRD	GSSQQLQGYI
110	120	130	140	150
PSNYVAEDRS	LQAEPWFFGA	IGRSDAEKQL	LYSENKTGSF	LIRESESQKG
160	170	180	190	200
EFSLSVLDGA	VVKHYRIKRL	DEGGFFLTRR	RIFSTLNEFV	SHYTKTSDGL
210	220	230	240	250
CVKLGKPCLK	IQVPAPFDLS	YKTVDQWEID	RNSIQLLKRL	GSGQFGEVWE
260	270	280	290	300
GLWNNTTPVA	VKTLKPGSMD	PNDFLREAQI	MKNLRHPKLI	QLYAVCTLED
310	320	330	340	350
PIYIITELMR	HGSLQEYLQN	DTGSKIHLTQ	QVDMAAQVAS	GMAYLESRNY
360	370	380	390	400
IHRDLAARNV	LVGEHNIYKV	ADFGLARVFK	VDNEDIYESR	HEIKLPVKWT
410	420	430	440	450
APEAIRSNKF	SIKSDVWSFG	ILLYEIITYG	KMPYSGMTGA	QVIQMLAQNY
460	470	480	490	500
RLPQPSNCPQ	QFYNIMLECW	NAEPKERPTF	ETLRWKLEDY	FETDSSYSDA
505
NNFIR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2793 Collapse assay details

Data source: Panorama

Official Gene Symbol: FRK
Peptide Modified Sequence: QLLY[+80.0]SENK
Modification Type: Phospho (Y)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 129
Peptide End: 136

CPTAC ID: CPTAC-2793
Peptide Molecular Mass: 1,073.4794
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Mammalian Cell Lysate
Submitting Laboratory: Johns Hopkins University / Pandey
Submitting Lab PI: Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Fusion Lumos
Internal Standard: 100fmol
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy nLC 1200 System
Column Packing: C18 2um 100A
Column Dimensions: 75um i.d. x 50cm
Flow Rate: 250nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	4.7	1.7	2.3	6.7	2.5	2.8	8.2	3	3.6	15	15	15
y5 (1+)	4.5	2.5	2.7	6.8	3.1	3	8.2	4	4	15	15	15
y4 (1+)	5	2.7	2.8	7.9	2.5	3	9.3	3.7	4.1	15	15	15
y2 (1+)	4.9	3	3.8	7.4	4.1	4	8.9	5.1	5.5	15	15	15
sum	4.6	2.1	2.7	6.9	2.8	3	8.3	3.5	4	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-3141 Collapse assay details

Data source: Panorama

Official Gene Symbol: FRK
Peptide Sequence: ERPTFETLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 476
Peptide End: 484

CPTAC ID: CPTAC-3141
Peptide Molecular Mass: 1,147.5986
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend