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Overview Data source: UniProt

Official Gene Symbol Other Aliases
FRK PTK5, RAK
Sequence Length (AA) Molecular Weight (Da)
505 58254
Protein Name
Tyrosine-protein kinase FRK
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSNICQRLWE YLEPYLPCLS TEADKSTVIE NPGALCSPQS QRHGHYFVAL
60 70 80 90 100
FDYQARTAED LSFRAGDKLQ VLDTLHEGWW FARHLEKRRD GSSQQLQGYI
110 120 130 140 150
PSNYVAEDRS LQAEPWFFGA IGRSDAEKQL LYSENKTGSF LIRESESQKG
160 170 180 190 200
EFSLSVLDGA VVKHYRIKRL DEGGFFLTRR RIFSTLNEFV SHYTKTSDGL
210 220 230 240 250
CVKLGKPCLK IQVPAPFDLS YKTVDQWEID RNSIQLLKRL GSGQFGEVWE
260 270 280 290 300
GLWNNTTPVA VKTLKPGSMD PNDFLREAQI MKNLRHPKLI QLYAVCTLED
310 320 330 340 350
PIYIITELMR HGSLQEYLQN DTGSKIHLTQ QVDMAAQVAS GMAYLESRNY
360 370 380 390 400
IHRDLAARNV LVGEHNIYKV ADFGLARVFK VDNEDIYESR HEIKLPVKWT
410 420 430 440 450
APEAIRSNKF SIKSDVWSFG ILLYEIITYG KMPYSGMTGA QVIQMLAQNY
460 470 480 490 500
RLPQPSNCPQ QFYNIMLECW NAEPKERPTF ETLRWKLEDY FETDSSYSDA
505
NNFIR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2793 Collapse assay details

Data source: Panorama

Official Gene Symbol
FRK
Peptide Modified Sequence
QLLY[+80.0]SENK
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
4
Peptide Start
129
Peptide End
136
CPTAC ID
CPTAC-2793
Peptide Molecular Mass
1,073.4794
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4.7 1.7 2.3 6.7 2.5 2.8 8.2 3 3.6 15 15 15
y5 (1+) 4.5 2.5 2.7 6.8 3.1 3 8.2 4 4 15 15 15
y4 (1+) 5 2.7 2.8 7.9 2.5 3 9.3 3.7 4.1 15 15 15
y2 (1+) 4.9 3 3.8 7.4 4.1 4 8.9 5.1 5.5 15 15 15
sum 4.6 2.1 2.7 6.9 2.8 3 8.3 3.5 4 15 15 15

Additional Resources and Comments